Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.7K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.7K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.3K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.3K
Cohesins02:20

Cohesins

5.5K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
5.5K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

5.4K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.4K
Microtubules in Signaling01:22

Microtubules in Signaling

2.1K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
2.1K
Role of Septins01:02

Role of Septins

2.1K
Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Development of the fluorescent probe CenSpark for labeling centrioles and cilia.

Nature chemical biology·2026
Same author

Development of the fluorescent probe CenSpark for labeling centrioles and cilia.

Nature chemical biology·2026
Same author

Critical constituents and assembly principles of centriole biogenesis in human cells.

Nature reviews. Molecular cell biology·2025
Same author

Multi-step implementation of meiotic crossover patterning.

bioRxiv : the preprint server for biology·2025
Same author

One-shot design of functional protein binders with BindCraft.

Nature·2025
Same author

BindCraft: one-shot design of functional protein binders.

bioRxiv : the preprint server for biology·2024

Related Experiment Video

Updated: Jan 14, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.7K

C. elegans SAS-1 ensures centriole integrity and ciliary function, and operates with SSNA-1.

Keshav Jha1, Alexander Woglar1, Coralie Busso1

  • 1Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland.

Plos Genetics
|October 22, 2025
PubMed
Summary

The microtubule-binding protein SAS-1 is crucial for maintaining centriole integrity in C. elegans, working with SSNA-1 to ensure proper organelle function and cilium activity.

More Related Videos

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

3.0K
Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
09:41

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

2.2K

Related Experiment Videos

Last Updated: Jan 14, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.7K
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

3.0K
Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
09:41

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

2.2K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Centrioles are essential microtubule-based organelles involved in cell signaling, motility, and division.
  • The SAS-1 protein in C. elegans is homologous to human C2CD3 and is implicated in centriole integrity, but its precise function is unclear.

Purpose of the Study:

  • To elucidate the role of SAS-1 in centriole assembly, integrity, and function.
  • To investigate the relationship between SAS-1 and SSNA-1 in centriole biogenesis and ciliary function.

Main Methods:

  • Generation and analysis of a SAS-1 null allele in C. elegans.
  • Ultra-Expressed Super-Resolution STED microscopy.
  • Molecular epistasis experiments and heterologous human cell assays.

Main Results:

  • SAS-1 is not required for centriole assembly initiation but is essential for maintaining centriole integrity throughout development.
  • SAS-1 localizes to the centriole transition zone and partially contributes to ciliary function.
  • SAS-1 is required for the localization of SSNA-1 to centrioles and the transition zone, and recruits SSNA-1 to microtubules.

Conclusions:

  • SAS-1, in conjunction with SSNA-1, plays a vital role in ensuring centriole integrity.
  • SAS-1 contributes to cilium function, highlighting its importance in microtubule-based organelles.