Use of Eco-friendly recipients for vitrification of Hyphessobrycon boulengery Spermatogonial cells

The aim of the study was to evaluate the efficiency of the gelatin and Hypromellose capsules as recipients to vitrification of lambari spermatogonial cells. In experiment 1, four protocols to vitrification (P1, P2, P3 and P4) were tested. The protocol P3 showed greater cell viability (25.42± 4.19%) compared to the other treatments. In experiment 2, evaluated the gelatine and hypromellose capsules in comparation with the cryotubes as recipients for vitrification. Variables as: concentration and viability cellular, mitochondrial activity, and oxidative stress were analyzed. Cryotubes obtained higher cellular concentration in comparation to the gelatine and hypromellose capsules, while it showed similarity in cellular viability with the gelatine capsule. Mitochondrial activity was not different among the treatments as the lipid peroxidation. Cryotubes and hypromellose capsule obtain highest levels of antioxidant capacity. These results suggest that biodegradables capsules have the capacity to preserved testicular tissue to obtain viable spermatogonial cells after vitrification and warming. In addition to play an important role as an alternative of vitrification recipients.