Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.1K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.1K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.6K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.6K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.3K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.3K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.1K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.1K
Nucleosome Remodeling02:54

Nucleosome Remodeling

10.8K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
10.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Filling the Void: Rapid Revascularization via Vasculogenic Assembly in Semi-synthetic Granular Hydrogel Grafts.

bioRxiv : the preprint server for biology·2026
Same author

3D-Printed Porous Titanium versus Polyetheretherketone Cages in Lumbar Interbody Fusion: A Prospective, Multicenter, Randomized Controlled Trial with Bone Mineral Density Stratification.

The spine journal : official journal of the North American Spine Society·2026
Same author

Perivascular Matrix Densification Dysregulates Angiogenesis and Activates Pro-Inflammatory Endothelial Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

What it Takes to Direct With a Doctor's Eye-Radiology Trailblazers, an <i>AJR</i> Podcast Series (Episode 12).

AJR. American journal of roentgenology·2026
Same author

The tumor microenvironment in triple negative breast cancer and a strategy to improve responses to immunotherapy using cryoablation and immunostimulants.

Cancer biology & therapy·2026
Same author

Optimizing Postoperative Sagittal Alignment: The Effect of Pedicle Screw Fixation in 540° Combined Surgery for Degenerative Cervical Disease.

Global spine journal·2026

Related Experiment Video

Updated: Jan 14, 2026

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

11.0K

Manipulation of the nucleoscaffold potentiates cellular reprogramming kinetics.

Benjamin A Yang1,2, Camila Vesga-Castro1,2, André Monteiro da Rocha3

  • 1Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

PNAS Nexus
|October 23, 2025
PubMed
Summary

The nuclear scaffold, specifically Lamin A/C, guards cell fate by maintaining silenced genes. Its loss accelerates reprogramming, while mutation causes senescence, highlighting its physical role in cell fate.

More Related Videos

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
13:23

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

Published on: February 20, 2012

20.4K
Author Spotlight: Improved Nucleofection for High-Efficiency Gene Delivery in Murine Subventricular Zone-Derived Neural Stem Cell Cultures
09:19

Author Spotlight: Improved Nucleofection for High-Efficiency Gene Delivery in Murine Subventricular Zone-Derived Neural Stem Cell Cultures

Published on: June 14, 2024

3.2K

Related Experiment Videos

Last Updated: Jan 14, 2026

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

11.0K
Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
13:23

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

Published on: February 20, 2012

20.4K
Author Spotlight: Improved Nucleofection for High-Efficiency Gene Delivery in Murine Subventricular Zone-Derived Neural Stem Cell Cultures
09:19

Author Spotlight: Improved Nucleofection for High-Efficiency Gene Delivery in Murine Subventricular Zone-Derived Neural Stem Cell Cultures

Published on: June 14, 2024

3.2K

Area of Science:

  • Cell Biology
  • Biophysics
  • Genetics

Background:

  • Somatic cell fate is determined by transcription factors and chromatin, maintained by silencing alternative fates via the nuclear scaffold.
  • Lamin A/C is a core component of the nuclear scaffold, crucial for nuclear structure and function.

Purpose of the Study:

  • To investigate the role of the nuclear scaffold, particularly Lamin A/C, in maintaining cell fate in human fibroblasts.
  • To compare the effects of Lamin A/C knockdown and progerin mutation on nuclear properties and cellular reprogramming.

Main Methods:

  • Assessed nuclear morphology and mechanical properties using microfluidic cellular squeezing.
  • Analyzed chromatin accessibility and gene expression changes following Lamin A/C manipulation.
  • Evaluated cellular reprogramming kinetics and senescence induction.

Main Results:

  • Lamin A/C deficiency/mutation disrupted nuclear morphology and mechanics.
  • Loss of Lamin A/C opened silenced heterochromatin and increased DNA accessibility.
  • Lamin A/C loss accelerated reprogramming; progerin mutation induced senescence and inhibited reprogramming genes.

Conclusions:

  • The nuclear scaffold, via Lamin A/C, physically safeguards cell fate.
  • Lamin A/C's mechanical and structural roles are critical for maintaining cellular identity and preventing senescence.