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Related Concept Videos

iChip01:24

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Related Experiment Video

Updated: Jul 8, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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Gut-on-a-Chip-Based Real-Time miRNA-21 Monitoring and Anti-Inflammatory Drug Evaluation.

Zhipeng Xu1, Jiemeng Ding2,3,4, Qi Meng2,3,4

  • 1Division of Clinical Medicine, School of Medicine & Population Health, Medical School, University of Sheffield, Sheffield S10 2RX, U.K.

ACS Sensors
|October 23, 2025
PubMed
Summary

We developed a gut-on-a-chip platform with a biosensor to detect miRNA-21, a biomarker for inflammatory bowel disease (IBD). This advanced model aids in monitoring inflammation and screening anti-inflammatory drugs.

Keywords:
MicroRNA-21drug screeningelectrochemical biosensorgut-on-a-chip (GOC)inflammation detection

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Area of Science:

  • Biomedical Engineering
  • Gastroenterology
  • Biosensor Technology

Background:

  • MicroRNA-21 (miRNA-21) is a key biomarker in inflammatory pathways, particularly relevant to gastrointestinal diseases like inflammatory bowel disease (IBD).
  • Dynamic miRNA-21 expression correlates with disease progression and treatment efficacy, highlighting its diagnostic and therapeutic potential.
  • Existing in vitro models lack the physiological relevance for accurate miRNA-21 monitoring and effective drug screening.

Purpose of the Study:

  • To develop an advanced gut-on-a-chip (GOC) platform integrated with an electrochemical biosensor for sensitive miRNA-21 detection.
  • To create a physiologically relevant in vitro model for monitoring intestinal inflammation and evaluating drug responses.

Main Methods:

  • Development of a GOC platform mimicking intestinal microenvironment with perfusion and mechanical stretching.
  • Integration of an electrochemical biosensor for high-sensitivity miRNA-21 quantification.
  • Establishment of an in vitro inflammation model using Caco-2 cells and pro-inflammatory stimuli.

Main Results:

  • The integrated biosensor achieved a wide linear detection range (1 × 10-15 to 1 × 10-10 M) for miRNA-21.
  • The GOC platform successfully monitored dynamic changes in miRNA-21 levels during induced inflammation.
  • The system demonstrated the correlation between miRNA-21 expression and inflammatory progression.
  • Proof-of-concept for evaluating anti-inflammatory drug efficacy using the platform was established.

Conclusions:

  • The developed GOC-biosensor platform offers a physiologically relevant system for sensitive miRNA-21 detection.
  • This platform enables dynamic monitoring of inflammation and serves as a valuable tool for drug screening in IBD research.
  • The system advances in vitro modeling for biomarker analysis and therapeutic evaluation in gastrointestinal diseases.