Mineralized Lipid Nanoparticles Containing Gadolinium Enable Integrated X-ray Fluorescence Computed Tomography...

Area of Science:

• Nanotechnology
• Biomedical Imaging
• Radiotherapy

Background:

• Glioblastoma multiforme (GBM) presents significant challenges due to its invasiveness and poor prognosis.
• Effective drug delivery across the blood-brain barrier (BBB) remains a major hurdle in GBM treatment.
• Current therapeutic strategies often lack precision and tumor-specific targeting.

Purpose of the Study:

• To develop a theranostic nanoplatform for enhanced glioblastoma treatment and imaging.
• To improve blood-brain barrier (BBB) penetration and tumor retention for glioblastoma multiforme (GBM).
• To enable dual-modality imaging (XFCT/CBCT) for radiotherapy guidance and dose amplification.

Main Methods:

• Fabrication of mineralized lipid vesicles encapsulating gadolinium ions (Gd3+) chelated by guanosine-5'-triphosphate (GTP).
• Evaluation of the nanoplatform's blood-brain barrier (BBB) penetration and intratumoral retention in glioblastoma models.
• Utilizing X-ray fluorescence computed tomography (XFCT) and cone-beam CT (CBCT) for imaging-guided radiotherapy.

Main Results:

• Gd-mineralized vesicles demonstrated enhanced BBB penetration and prolonged tumor retention.
• The nanoplatform enabled high-resolution, high-contrast mapping of intratumoral gadolinium distribution.
• Radiotherapy guided by XFCT/CBCT imaging showed enhanced efficacy through localized dose amplification.

Conclusions:

• GTP-mediated Gd mineralization in liposomes offers a novel theranostic approach for glioblastoma.
• This dual-modality imaging system enhances precision in radiotherapy delivery and radiosensitization.
• The developed nanoplatform presents a versatile and translational strategy for GBM precision medicine.