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Investigation of potential biomarkers associated with relapse in cervical cancer: miRNA-320a-3p and miRNA-136-5p
Melike Pekyürek Varan1, Gökhan Yaprak2, Kübra Gündüz3
1Department of Radiation Oncology, Kartal Dr. Lütfi Kırdar City Hospital, Istanbul, Turkey. melikepekyurek@gmail.com.
Purpose:
Micro ribonucleic acids (miRNAs) are short non-coding RNA molecules that control the expression of target mRNAs. Many miRNAs are dysregulated in various cancers, acting as tumor suppressors or oncogenes. This study aims to investigate the regulatory status of miRNA-320a-3p and miRNA-136-5p in cervical cancer development as well as their predictive significance for treatment response and disease recurrence.
Materials And Methods:
Included in the study were 51 cervical cancer patients and 19 healthy controls. Expression levels of miRNA-320a-3p and miRNA-136-5p were detected using miRNA-specific quantitative real-time polymerase chain reaction (PCR). The relative expression of miRNAs was calculated using the 2-∆∆Ct method.
Results:
miRNA-136-5p was downregulated in the cervical cancer patients compared to the controls (fold change: -3.06, p = 0.000239). In the case group, upregulation of miRNA-320a-3p was significantly associated with a poor treatment response (fold change: 1.88, p = 0.0264). Additionally, higher expression of miRNA-320a-3p was observed in patients with locoregionally confined recurrence both in the evaluation based on initial recurrence patterns (fold change: 1.38, p = 0.0341) and at last follow-up (fold change: 2.56, p = 0.000582).
Conclusion:
miRNA-136-5p can be proposed as a biomarker in cervical tumorigenesis, while miRNA-320a-3p can be used for treatment response and locoregional recurrence prediction.
Insights
Micro ribonucleic acid-136-5p (miRNA-136-5p) is downregulated in cervical cancer, suggesting it as a biomarker. Micro ribonucleic acid-320a-3p (miRNA-320a-3p) upregulation predicts poor treatment response and recurrence risk.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Micro ribonucleic acids (miRNAs) are key regulators of gene expression.
- Aberrant miRNA expression is implicated in various cancers, including cervical cancer.
- Specific miRNAs may serve as biomarkers for cancer development and progression.
Purpose of the Study:
- To investigate the expression levels of miRNA-320a-3p and miRNA-136-5p in cervical cancer.
- To evaluate the potential of these miRNAs as biomarkers for cervical cancer diagnosis.
- To assess their predictive significance for treatment response and disease recurrence.
Main Methods:
- Quantitative real-time polymerase chain reaction (PCR) was used to measure miRNA expression.
- Expression levels were analyzed in 51 cervical cancer patients and 19 healthy controls.
- The 2-∆∆Ct method was employed for relative quantification.
Main Results:
- miRNA-136-5p was significantly downregulated in cervical cancer patients (fold change: -3.06, p=0.000239).
- Upregulation of miRNA-320a-3p correlated with poor treatment response (fold change: 1.88, p=0.0264).
- Higher miRNA-320a-3p expression was associated with locoregional recurrence (p=0.0341 and p=0.000582).
Conclusions:
- miRNA-136-5p shows potential as a biomarker for cervical tumorigenesis.
- miRNA-320a-3p may serve as a predictive biomarker for treatment response and locoregional recurrence in cervical cancer.
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