Related Experiment Video
Updated: Jan 14, 2026

Light-Controlled Fermentations for Microbial Chemical and Protein Production
Published on: March 22, 2022
Recent advances in microbial fermentation for the production of leghemoglobin
Zixin Yao1,2,3,4, Jianbin Weng1,2,3,4, Mengxin Wang1,2,3,4
1Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China.
Abstract:
Leghemoglobin (LegH) is a monomer heme-binding protein in legume rhizomes, which possesses essential functions in cellular oxygen transport and provision of bioavailable iron. Meanwhile, it has great potential application in food additive for the promising artificial meat, endowing the products realistic color and taste. Compared with the traditional extraction method, the production of LegH by microbial biosynthesis has advantages in short production period, low cost and high yield. In this review, we summarize the recent strategies for the optimization of LegH expression in different microorganisms, the metabolic regulation of heme synthetic pathway, and the progress in the improvement of fermentation process. Compared with the previous reviews, this review covers numerous prokaryotic and eukaryotic microorganisms used for LegH production, and also takes into account the improvement of LegH secretory expressional level. Additionally, this review summarizes the key technological breakthroughs and existing challenges in the field of current LegH microbial synthesis, providing a reference for the subsequent industrial production and application of LegH and other high-value-added hemoproteins.
More Related Videos
06:24Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
14:53Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Related Concept Videos
Microbial Fermentation
Fermentation
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Fates of Pyruvate
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
Microorganisms in Agriculture and Food industry
Amino Acid Catabolism