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Preparation of Samples for Electron Microscopy01:20

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Related Experiment Video

Updated: Jan 14, 2026

Comprehensive Characterization of Extended Defects in Semiconductor Materials by a Scanning Electron Microscope
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Efficient and Robust SEM Image Denoising for Wafer Defect Inspection.

Hyunwoong Bae1, Jaeseok Byun2, Yongwoo Lee3

  • 1Interdisciplinary Program in Artificial Intelligence, Seoul National University, Gwanak-ro, South Seoul 08826, Korea.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|October 23, 2025
PubMed
Summary
This summary is machine-generated.

We developed Relaxed Noise2Noise with Input dropout (ReNIn) for scanning electron microscopy (SEM) denoising. ReNIn offers efficient training and improved generalization for wafer defect inspection.

Keywords:
SEMdeep learningdenoisingdenoising for inspection

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Area of Science:

  • Materials Science
  • Computer Science
  • Image Processing

Background:

  • Scanning electron microscopy (SEM) noise hinders accurate wafer defect inspection.
  • Current deep learning denoising methods for SEM suffer from inefficiency and poor generalization to novel image structures.

Purpose of the Study:

  • To introduce an efficient and robust deep learning-based denoising method for SEM images.
  • To enhance the generalization capability of denoising models for unseen image structures.
  • To improve downstream wafer inspection tasks, such as circle detection.

Main Methods:

  • Proposed Relaxed Noise2Noise (ReNIn) framework combining a relaxed noise2noise approach with input dropout.
  • Relaxed Noise2Noise component optimizes the trade-off between denoising performance and data collection cost.
  • Input dropout technique enhances model generalization to diverse image structures.

Main Results:

  • ReNIn achieves competitive denoising performance with significantly reduced training data collection costs compared to supervised methods.
  • Input dropout improves performance on structurally novel images without degrading performance on familiar structures.
  • The method enhances circle detection accuracy in downstream inspection tasks.

Conclusions:

  • ReNIn offers an efficient solution for SEM image denoising, balancing performance and data acquisition costs.
  • The proposed method demonstrates superior generalization capabilities for SEM image analysis.
  • ReNIn effectively supports critical wafer inspection processes by improving defect detection accuracy.