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A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
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Published on: April 28, 2019

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Association of intermediate monocytes with dengue severity among the pediatric population of Odisha, India

Manoj Kumar Dash1, Sagnika Samal1, Shailesh Rout1

  • 1School of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Bhubaneswar, Odisha, 751024, India.

Scientific Reports
|October 23, 2025
PubMed

Insights

Increased intermediate monocytes (IM) in children with dengue virus (DENV) infection may indicate disease severity. This study analyzed monocyte subpopulations and DENV protein interactions, identifying IM as potential severity markers.

Area of Science:

  • * Immunology
  • * Virology
  • * Computational Biology

Background:

  • * Dengue virus (DENV) infection in children presents with diverse clinical outcomes.
  • * Monocytes play a crucial role in the innate immune response to DENV.
  • * Understanding monocyte subpopulations can provide insights into DENV pathogenesis.

Purpose of the Study:

  • * To analyze monocyte subpopulations (CD14, CD16) in pediatric DENV patients.
  • * To correlate monocyte profiles with clinical DENV classifications (DF, DWS, SD).
  • * To investigate potential molecular interactions between host monocytes and DENV proteins.

Main Methods:

  • * Immunophenotyping of monocytes using CD14 and CD16 markers in 120 pediatric DENV patients.
  • * Categorization of patients into Dengue Fever (DF), Dengue with Warning Signs (DWS), and Severe Dengue (SD).
  • * Molecular docking simulations (HADDOCK 2.4) to assess CD14/CD16 and DENV protein interactions.

Main Results:

  • * Intermediate monocytes (IM-CD14+CD16+) were significantly higher in DWS & SD cases (30.86%) compared to DF cases (9.89%).
  • * Classical monocytes (CM) were more prevalent in DF, while Non-classical monocytes (NCM) showed no significant difference.
  • * Molecular docking indicated potential binding of CD14 and CD16 to DENV capsid and envelope proteins.

Conclusions:

  • * Intermediate monocytes (IM) are significantly elevated in children with severe DENV infection.
  • * IM may serve as a potential host-derived biomarker for predicting DENV severity in pediatric cases.
  • * In silico findings suggest direct interactions between host monocyte markers and DENV proteins.

Keywords:
Classical monocytesDengue virusIntermediate monocytesMonocyte subpopulationsNon-classical monocytes

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