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Updated: Jan 14, 2026
Author Spotlight: Advancements and Challenges in Hepatitis B Virus Detection
Published on: December 15, 2023
Effect of Temperature on Detection of Hepatitis B Surface Antigen by Gingival Swab
Hira Tariq1, Mateen Izhar2, Namra Mahmood3
1Pathology/Microbiology, Avicenna Medical College, Lahore, PAK.
Abstract:
Objective The objective of the study was to determine the effect of two temperature points (27-32 °C and 50 °C) on the detection of Hepatitis B surface antigen (HBsAg) by gingival swab. Method The study was conducted in 2024 in Shaikh Zayed Hospital, Lahore, Pakistan. It was a non-randomized controlled study done over a period of one year. The study included 138 known positive cases of hepatitis B and 138 known negative cases, as validated by serum enzyme-linked immunosorbent assay (ELISA). Patients with mouth ulcers, gum bleeding, or gingivitis were excluded. Two gingival swabs were obtained from each patient. The swabs were kept in phosphate-buffered saline solution at two different temperatures (27-32 °C and 50 °C) for 24 hours. Automated ELISA performed HBsAg detection on both samples from each patient. Results Out of 138 known hepatitis B positive individuals, 108 swabs tested positive for HBsAg at 50 °C and 84 at 27-32 °C. Swabs of all 138 known hepatitis B-negative patients were negative at both 50 °C and 27-32 °C. Conclusion HBsAg is more likely to be detected by swab samples at higher temperatures, and no cold chain is required to transport these swab samples.
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