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Related Concept Videos

Response Surface Methodology01:16

Response Surface Methodology

604
Response Surface Methodology (RSM) is a collection of statistical and mathematical techniques used to develop, improve, and optimize processes. It is particularly valuable when many input variables or factors potentially influence a response variable.
The process of RSM involves several key steps:
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Developing and Validating a Robust RP-HPLC Method for Metoclopramide and Camylofin Simultaneous Analysis Using

Ahmed Hussain Jawhari1, Zeinhom H Mohamed1

  • 1Department of Physical Sciences, Chemistry Division, College of Science, Jazan University, P.O. Box. 114, Jazan 45142, Saudi Arabia.

International Journal of Analytical Chemistry
|October 24, 2025
PubMed
Summary

A new reversed-phase high-performance liquid chromatography (RP-HPLC) method accurately quantifies metoclopramide (MET) and camylofin (CAM) together. This validated method is suitable for quality control of pharmaceutical formulations.

Keywords:
HPLC method developmentcamylofinchromatographic separationmetoclopramideresponse surface methodology

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Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Analysis

Background:

  • Metoclopramide (MET) and camylofin (CAM) are commonly used drugs.
  • Accurate simultaneous quantification of these drugs in dosage forms is essential for quality control.

Purpose of the Study:

  • To develop and validate a stable reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of MET and CAM.
  • To optimize the method using response surface methodology (RSM) considering the physicochemical properties of both drugs.

Main Methods:

  • RP-HPLC method development and optimization using Design Expert Software 13.
  • Utilized a phenyl-hexyl column with an isocratic mobile phase of methanol and ammonium acetate buffer (pH 3.5).
  • Method validation including linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and recovery studies.

Main Results:

  • The developed RP-HPLC method demonstrated excellent linearity (R² > 0.999) for both MET and CAM.
  • Achieved low LOD (0.23 μg/mL for MET, 0.15 μg/mL for CAM) and LOQ (0.35 μg/mL for MET, 0.42 μg/mL for CAM).
  • High recovery (98.2%-101.5%) and excellent precision (RSD < 2%) were obtained. The method was successfully applied to commercial tablet formulations.

Conclusions:

  • A validated, sensitive, accurate, and efficient RP-HPLC method for simultaneous determination of MET and CAM in pharmaceutical dosage forms has been established.
  • The method is suitable for routine quality control and regulatory analyses of drug formulations containing both metoclopramide and camylofin.