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Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
Published on: May 2, 2018
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Automatic Follicle Counting From Ultrasound Images of Ovaries Using MARDSE-UNET Model.
Debasmita Saha1, Ardhendu Mandal2, Akhil Kumar Das3
1Department of Computer Science, University of Gour Banga, Malda, West Bengal, India.
Ultrasonic Imaging
|October 24, 2025
Summary
This study introduces an automated system for detecting ovarian follicles in ultrasound images, crucial for diagnosing reproductive health issues. The novel MARDSE-UNet model significantly improves accuracy in identifying these vital structures.
Area of Science:
- Medical Imaging
- Artificial Intelligence in Medicine
- Reproductive Endocrinology
Background:
- Ovarian structure detection in ultrasound is vital for gynecological and reproductive medicine.
- Automated systems can assist physicians in interpreting complex ultrasound images.
- Accurate follicle identification is key for diagnosing conditions like PCOS and infertility.
Purpose of the Study:
- To develop and evaluate a CNN-based object detector for segmenting and counting ovarian follicles.
- To enhance follicle detection performance using an integrated deep learning architecture.
- To provide a comprehensive tool for reproductive health diagnostics.
Main Methods:
- Development of the Multi-Attention Residual Dilated UNet with Squeeze and Excitation (MARDSE-UNet) model.
- Integration of residual UNet, dilated UNet, and squeeze-and-excitation blocks.
- Utilized the USOVA3D dataset with 5-fold cross-validation for rigorous testing.
Main Results:
- MARDSE-UNet achieved high performance: 98.69% accuracy, 97.89% precision, 97.7% recall, 86.97% F1-score, and 95.66% IoU.
- Novel preprocessing and post-processing stages improved noise reduction and feature extraction.
- The model outperformed traditional CNNs and state-of-the-art methods.
Conclusions:
- The MARDSE-UNet model demonstrates superior performance in automated ovarian follicle detection.
- The system offers a valuable tool for diagnosing various reproductive health conditions.
- Further development can enhance clinical applications in gynecological imaging.

