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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

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Related Experiment Video

Updated: May 11, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
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A Robust, High-Titer, Semi-Automated, and In-Culture Antibody-Capturing Transient CHO Platform Technology.

Lauren Gebhardt1, Molica Abel1, Jing Zhou1

  • 1BioMedicine Design, Preclinical and Translational Sciences, Pfizer Research and Development, 610 MainStreet, Cambridge, MA 02139, USA.

Antibodies (Basel, Switzerland)
|October 24, 2025
PubMed
Summary
This summary is machine-generated.

Advances in antibody discovery necessitate rapid, large-scale production. A new semi-automated workflow enables the efficient generation of purified antibodies for functional screening and molecular assessment.

Keywords:
AKTA PureTMCHO4Tx®GenScript AmMag™ SA-Plusin-culture antibody capturingmagnetic ProA beadssemi-automationtransient gene expression

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunology

Background:

  • Machine learning advances in antibody discovery present production challenges.
  • Demand exists for rapid, 10-milligram quantities of diverse antibodies for screening.

Purpose of the Study:

  • Develop a semi-automated workflow to bridge high-throughput screening and custom-scale antibody production.
  • Integrate novel technologies to meet the demand for rapid antibody generation.

Main Methods:

  • Utilized a high-titer transient expression system (CHO4Tx®) for high antibody yields (200 mg/L+).
  • Implemented in-culture magnetic ProA bead capturing to streamline purification.
  • Employed semi-automated magnetic bead elution (GenScript AmMag™ SA Plus) and automated buffer exchange (AKTA Pure™).

Main Results:

  • Achieved ~20 mg crude material per 100 mL flask with high throughput (19 constructs/run).
  • Reduced production timelines by eliminating centrifugation, filtration, and column loading steps.
  • Enabled processing of 12 constructs for elution within 1 hour and 19 samples for buffer exchange per run.

Conclusions:

  • The new platform is robust for routine antibody production.
  • Generated antibodies are of sufficient quality and quantity for cell-based assays and biophysical characterization.