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Standardizing caloric restriction for preclinical cancer research
Jenna L Jacoby1, Ali Calderon Aparicio1, Noëlle B François1
1Department of Radiation Oncology, Sidney Kimmel Comprehensive Cancer Center Thomas Jefferson University, Philadelphia, PA, USA.
Biotechniques
|October 24, 2025
Summary
Caloric restriction (CR) improves cancer radiotherapy outcomes in mice by promoting apoptosis. This study introduces a novel, reproducible CR protocol for cancer research, enabling safe and effective caloric reduction in animal models.
Area of Science:
- Oncology
- Metabolic Research
- Animal Models
Background:
- Metabolic syndrome is prevalent in cancer patients, yet often overlooked in treatment.
- Caloric restriction (CR) shows promise for enhancing cancer treatment response.
- Previous CR preclinical studies lack standardized protocols, hindering clinical translation.
Purpose of the Study:
- To demonstrate that a CR diet enhances radiotherapy outcomes in mice.
- To propose a novel, reproducible, and translationally relevant CR protocol for cancer studies.
Main Methods:
- Developed a two-week CR protocol involving individual caloric intake measurement and gradual weaning.
- Achieved a safe 30% caloric reduction over two weeks.
- Integrated individualized intake measurement, gradual weaning, and real-time monitoring.
Main Results:
- The CR diet enhanced radiotherapy outcomes in mice.
- CR promoted apoptosis and downregulated cell survival pathways.
- The proposed protocol ensures a safe and reproducible 30% caloric reduction.
Conclusions:
- A standardized CR protocol can improve radiotherapy efficacy in preclinical cancer models.
- The developed protocol offers a reproducible framework for future cancer research.
- This approach facilitates the clinical translation of CR as an adjuvant cancer therapy.
