microRNA modulates aphid phototaxis in response to yellow sticky traps

Colored sticky traps are widely used for aphid population monitoring and control based on phototaxis. However, the underlying molecular mechanisms remain unclear.

Here, we explored microRNA (miRNA) regulation of phototaxis in the peach aphid (Myzus persicae), a major agricultural pest worldwide. Behavioral assays revealed that M. persicae consistently preferred yellow when tested using color cards of specific wavelengths under varying heights and time conditions. Small RNA sequencing identified significant downregulation of miR-13c in aphids attracted to yellow cards. Functional validation confirmed that miR-13c overexpression reduced this yellow preference in M. persicae. By combining RNA sequencing, target prediction algorithms, dual-luciferase reporter, and RNA pull-down assays, we demonstrated that miR-13c directly targets the transient receptor potential protein (MpTRP). MpTRP silencing triggered phototaxis impairment similar to that caused by miR-13c overexpression, both of which reduced yellow preference in M. persicae.

These findings establish the miR-13c-MpTRP axis as a key molecular mechanism regulating aphid phototaxis, providing evidence of miRNA-mediated light-seeking behavior in insects. The study proposes a foundational framework for developing RNA interference-based biocontrol strategies targeting miRNA-photoreception interactions, offering a sustainable alternative to chemical pesticides. © 2025 Society of Chemical Industry.