Mesenchymal Stem Cell-Inspired Microneedle Platform for NIR-responsive Immunomodulation and Accelerated Chronic Wound...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Wound Healing

Background:

• Chronic diabetic wounds pose significant clinical challenges due to persistent inflammation and poor healing.
• Mesenchymal stem cells (MSCs) secrete exosomes that modulate the tissue microenvironment for regeneration.

Purpose of the Study:

• To develop a biomimetic microneedle (MSCi@MN) platform for enhanced chronic diabetic wound treatment.
• To leverage MSC-derived exosomes and targeted drug delivery for improved therapeutic outcomes.

Main Methods:

• Fabrication of a dual-compartment microneedle platform containing MSC-derived nanovesicles conjugated with polydeoxyribonucleotide (NV-DNA) and MXene nanoparticles.
• Utilized near-infrared (NIR) irradiation for controlled release of NV-DNA and activation of photothermal properties.
• Investigated immunomodulatory effects, transcriptomic changes, and wound healing efficacy in diabetic animal models.

Main Results:

• MSCi@MN facilitated M2 macrophage polarization and activated tolerogenic dendritic cells, creating a regenerative microenvironment.
• Transcriptomic analysis confirmed NV-DNA promoted angiogenesis, proliferation, and extracellular matrix remodeling.
• MSCi@MN significantly accelerated wound repair, reduced inflammation, enhanced angiogenesis, and restored skin appendages in diabetic models without adverse effects.

Conclusions:

• The MSC-inspired microneedle platform offers a promising strategy for chronic wound management.
• This approach integrates controlled release with potent immunoregenerative capabilities for advanced regenerative medicine applications.