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Updated: Jan 14, 2026
Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
Published on: February 18, 2014
Protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometry
Matias Cardenas1, Sasha Compton1, Ashkon Hunt1
1Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Abstract:
Recent work has shown that major histocompatibility complex (MHC) class II is a functional receptor for some influenza A virus (FLUAV) subtypes. Here, we present a protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometry. We describe steps for producing recombinant human leukocyte antigen DR isotype (HLA-DR) MHC class II using a baculovirus expression system. The HLA-DR antigen is then modified for its utilization as a biolayer interferometry (BLI) probe for kinetic experiments using whole FLUAV viruses. This protocol allows for the screening of different FLUAV subtypes and their affinity for MHC class II.
