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Published on: May 1, 2021
Identification and characterization of Staphylococcus spp. Isolated in co-infection with respiratory viruses from
Kelliane Martins de Araujo1, Marcos de Oliveira Cunha1, Vivian Maria Cordeiro1
1Universidade Federal de Goiás, Institute of Tropical Pathology and Public Health, Microorganism Biotechnology Laboratory - LBMIc, Goiânia, GO, Brazil.
Abstract:
Healthcare-associated infections are among the most significant complications in hospitalized patients, posing a major challenge due to the antimicrobial resistance of pathogenic agents such as Staphylococcus spp. The study aims to identify and evaluate the phenotypic and molecular resistance profile of Staphylococcus spp. in co-infection with respiratory viruses, including COVID-19, as a respiratory virus, in samples from children admitted to ICUs. Nasopharyngeal samples from the biorepository were stored at -80 °C in medium containing gentamicin and amphotericin B. Bacterial strains were isolated, and antibiograms were performed using the Kirby-Bauer method with antimicrobials specific to Staphylococcus spp. and the method of evaluating molecular resistance, carrying out the amplification of resistance genes, using specific oligonucleotides. A multidrug-resistant profile was observed in Staphylococcus spp., highlighting the need for monitoring to ensure appropriate treatment. Antimicrobial resistance emphasized the importance of strict control over antibiotic use in hospital environments. This study contributes to the understanding of antimicrobial resistance in bacterial co-infections, providing insights for more effective treatments and HAI control strategies.
Insights
Healthcare-associated infections (HAIs) are a major concern. This study found Staphylococcus species exhibit multidrug resistance, especially when co-infecting with respiratory viruses like COVID-19 in children.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Public Health
Background:
- Healthcare-associated infections (HAIs) are a significant threat to hospitalized patients.
- Antimicrobial resistance (AMR) in pathogens like Staphylococcus species complicates treatment.
- Co-infections with respiratory viruses, including COVID-19, can exacerbate bacterial infections.
Purpose of the Study:
- To determine the phenotypic and molecular antimicrobial resistance profiles of Staphylococcus species.
- To investigate Staphylococcus spp. co-infections with respiratory viruses in pediatric ICU patients.
- To assess the impact of viral co-infections on bacterial AMR.
Main Methods:
- Isolation and identification of Staphylococcus species from nasopharyngeal samples.
- Phenotypic antimicrobial susceptibility testing using the Kirby-Bauer method.
- Molecular detection of antimicrobial resistance genes via oligonucleotide amplification.
Main Results:
- A multidrug-resistant profile was observed in Staphylococcus species isolates.
- Specific resistance genes were identified, confirming molecular resistance.
- The study highlights the prevalence of resistant Staphylococcus spp. in pediatric ICU patients with viral respiratory infections.
Conclusions:
- Staphylococcus species demonstrate significant multidrug resistance, particularly in co-infections with respiratory viruses.
- Continuous monitoring of AMR and strict antibiotic stewardship in hospitals are crucial.
- Findings provide insights for improved HAI control and treatment strategies against resistant bacteria.
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