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Updated: Jan 6, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Gut-lung axis and microbiome interplay in type 2 high asthma
1College of Pharmacy, Al Ain University, Abu Dhabi, United Arab Emirates.
Introduction:
Type-2-high (T2-high) asthma remains the most prevalent phenotype of this chronic airway disease. Accumulating evidence implicates the gut-lung microbiome axis as the principal mechanism underlying T2-immune modulation, influencing disease trajectory and therapeutic efficacy.
Areas Covered:
Following Ferrari's narrative-review framework, a three-stage search of PubMed/MEDLINE, Embase, Scopus, Web of Science, the Cochrane Library and trial registries (2010-March 2025) was conducted. This review integrates a decade of human and animal investigations in genomics, transcriptomics, metabolomics, and microbiomics. It elucidates cooperative cellular networks in T2-high asthma, traces the hematogenous, lymphatic, and neural conduits conveying gut-derived metabolites the airways, and examines the roles of barrier integrity. The review also included dietary modulation strategies ranging from fiber enrichment and Mediterranean dietary patterns to excessive ultra-processed food intake.
Expert Opinion:
Near-term translation will pair ICS/LABA/biologics with diet/postbiotics and validated, low-biomass-aware multi-omic panels; adequately powered RCTs and contamination-controlled pipelines are prerequisites for routine care.
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