Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Stability01:53

RNA Stability

35.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.6K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

3.4K
3.4K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

6.5K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.5K
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.4K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.4K
Pharmaceutical Alternatives: Stability-Related Therapeutic Nonequivalence01:22

Pharmaceutical Alternatives: Stability-Related Therapeutic Nonequivalence

177
Generic intravenous (IV) drugs are considered bioequivalent to their branded counterparts due to their 100% bioavailability upon administration. However, variations in stability among different drug products can significantly influence their therapeutic performance, even if they are pharmaceutically equivalent.Cefuroxime, a prophylactic antimicrobial, is often used as a single-dose IV injection for patients undergoing coronary artery bypass grafting surgery. A 3 g dose typically provides...
177
Translational Regulation01:29

Translational Regulation

531
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
531

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An experimental study on the role of naloxone in facilitating alcohol metabolism in acute alcohol intoxication rats.

Future science OA·2026
Same author

Lipid phase behavior governs microstructural retention and sustained α-tocopherol release in pullulan composite films for walnut kernel preservation.

Food research international (Ottawa, Ont.)·2026
Same author

Author Correction: CAF-derived GLUT1 and its role in modulating ovarian cancer progression: a multi-dimensional analysis of the tumor microenvironment.

Communications biology·2026
Same author

Recent Advances of Atomic/Molecular Layer Deposition Engineering Silicon Interface for Lithium-Ion Batteries.

Nano-micro letters·2026
Same author

Discovery of furan-trimethoxyphenyl hybrids as novel colchicine-binding site inhibitors that induce mitotic catastrophe with potent anti-acute myeloid leukemia effect.

Bioorganic chemistry·2026
Same author

Exploration of key stress granule-related genes in chronic sinusitis based on transcriptomics.

Scientific reports·2026

Related Experiment Video

Updated: Jan 14, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

1.0K

mRNA Stability: An Unresolved Challenge for Broad Therapeutic Applications.

Yiming Wang1,2,3, Xiaoxue Wang1,2,3, Yuan Lu1,2,3

  • 1Department of Chemical Engineering, Tsinghua University, Beijing, China.

Biotechnology Journal
|October 27, 2025
PubMed
Summary

Messenger RNA (mRNA) instability hinders its therapeutic use. This review covers strategies like sequence optimization, structural modifications, and delivery systems to enhance mRNA stability for diverse disease treatments.

Keywords:
mRNA deliverymRNA designmRNA modificationmRNA stabilitymRNA structure

More Related Videos

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents
06:51

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

Published on: August 10, 2018

8.1K
A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
06:53

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

Published on: December 26, 2015

13.0K

Related Experiment Videos

Last Updated: Jan 14, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

1.0K
Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents
06:51

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

Published on: August 10, 2018

8.1K
A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
06:53

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

Published on: December 26, 2015

13.0K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Therapeutics

Background:

  • Messenger RNA (mRNA) holds significant therapeutic promise for various diseases, including infectious diseases, cancers, and rare conditions.
  • The single-stranded nature of mRNA makes it susceptible to enzyme-mediated degradation, posing a major challenge to its clinical application.
  • Enhancing mRNA stability is critical for unlocking its full therapeutic potential.

Purpose of the Study:

  • To review current strategies for improving mRNA stability.
  • To explore methods for mitigating mRNA degradation.
  • To discuss the future outlook of mRNA technology in biotechnology.

Main Methods:

  • Review of literature on mRNA stabilization techniques.
  • Analysis of sequence and structural optimization methods for mRNA.
  • Exploration of RNA modifications and novel mRNA structures (e.g., circular mRNA, self-amplifying RNA).
  • Discussion of mRNA delivery systems and their role in stability.

Main Results:

  • Strategies to enhance mRNA stability include enzyme removal, protective substances, optimized storage, and transport.
  • Sequence optimization of 5' UTR, ORF, and 3' UTR, along with RNA modifications, significantly improves stability.
  • Novel mRNA structures like circular and self-amplifying RNA offer advanced stabilization approaches.
  • Delivery materials also contribute to improved mRNA stability.

Conclusions:

  • Optimizing mRNA stability is achievable through various chemical, structural, and delivery-based strategies.
  • Continued advancements in mRNA technology are paving the way for its broader application in biotechnology.
  • mRNA is poised to become a key player in versatile biotechnological fields.