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Updated: Jan 14, 2026

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Role of TPD52 in Endometrial Cancer: Impact on EMT and the PI3K/AKT and ERK/MAPK Signaling

Lu Miao1, Buze Chen1, Linlin Li1

  • 1Department of Gynecology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221009, Jiangsu, China.

Protein and Peptide Letters
|October 27, 2025
PubMed
Abstract

Insights

Tumor protein D52 (TPD52) drives endometrial carcinoma (EC) progression by promoting epithelial-mesenchymal transition (EMT) and activating key signaling pathways. Targeting TPD52 presents a potential therapeutic strategy for EC.

Area of Science:

  • Oncology
  • Molecular Biology

Background:

  • Endometrial carcinoma (EC) incidence and mortality are increasing, with limited therapeutic targets.
  • Tumor protein D52 (TPD52) is investigated for its role in EC pathogenesis.

Purpose of the Study:

  • To define the oncogenic role of TPD52 in EC.
  • To elucidate the mechanism of TPD52 action, focusing on epithelial-mesenchymal transition (EMT) and PI3K/AKT/ERK/MAPK signaling.

Main Methods:

  • TPD52 expression assessed via immunohistochemistry in EC and benign tissues.
  • In vitro and in vivo experiments using siRNA and overexpression plasmids in EC cell lines (Ishikawa, HEC-1-A).
  • Evaluated effects of TPD52 knockdown/overexpression on cell proliferation, migration, invasion, and EMT.

Main Results:

  • TPD52 significantly upregulated in EC tissues compared to benign tissues.
  • TPD52 knockdown inhibited EC cell proliferation, migration, and invasion.
  • TPD52 overexpression enhanced these processes and promoted EMT.
  • TPD52 activates PI3K/AKT and ERK/MAPK signaling pathways.

Conclusions:

  • TPD52 acts as an oncoprotein in EC, driving progression via EMT.
  • TPD52 facilitates EMT through dual PI3K/AKT and ERK/MAPK signaling.
  • TPD52 is a potential therapeutic target for EC, requiring further clinical investigation.
Keywords:
ERK/MAPK signaling pathwayEndometrial cancerPI3K/AKT signaling pathwayepithelial-mesenchymal transitiontherapeutic targets.tumor protein D52

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