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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
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Multi-exposure speckle imaging through an optical fiber bundle.

Logan Parker1, Shaun A Englemann1, Alankrit Tomar1

  • 1The University of Texas at Austin, Department of Biomedical Engineering, Austin, Texas, United States.

Journal of Biomedical Optics
|October 27, 2025
PubMed
Summary

Multi-exposure speckle imaging (MESI) can now be performed through optical fiber bundles, enabling blood flow measurements in challenging environments. This technique shows accuracy comparable to traditional free-space MESI, expanding its clinical applications.

Keywords:
flowoptical imagingspecklespeckle contrastspeckle imaging

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Area of Science:

  • Biomedical optics
  • Medical imaging
  • Hemodynamics

Background:

  • Multi-exposure speckle imaging (MESI) is a label-free optical technique for visualizing and quantifying blood flow.
  • Accurate perfusion measurements are crucial for surgical guidance, treatment monitoring, and disease diagnosis.

Purpose of the Study:

  • To demonstrate the feasibility of using optical fiber bundles for MESI.
  • To assess the accuracy of fiber-bundle MESI compared to free-space MESI.
  • To explore applications in areas like endoscopy or confined spaces.

Main Methods:

  • Simultaneous acquisition of MESI data from a flow phantom and *in vivo* mouse cortex using both free-space and fiber-bundle setups.
  • Comparison of measurements using the Pearson correlation coefficient (R²).

Main Results:

  • High correlation (R² > 0.99) between fiber-bundle and free-space MESI for flow phantoms across a wide range of flow rates (1-100 μL/min).
  • Strong agreement (R² = 0.970) for *in vivo* measurements in mouse cortex vessels and parenchyma.
  • Significant correlation (R² > 0.90) observed during stroke modeling.

Conclusions:

  • Fiber-bundle MESI is a feasible and accurate method for blood flow measurement.
  • This technique offers comparable performance to free-space MESI.
  • It expands the potential applications of MESI, particularly in minimally invasive procedures and challenging anatomical locations.