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Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

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Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
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Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
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Updated: Jan 14, 2026

Author Spotlight: A Model to Study the Systemic and Local Dynamics of CD8+ T Cells During LN Metastasis
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Tumor-driven stromal reprogramming in the pre-metastatic lymph node.

Michelle Piquet1, David A Ruddy1, Viviana Cremasco2

  • 1Oncology Innovative Targets and Technologies, Novartis, Cambridge, MA, 02139, USA.

F1000Research
|October 27, 2025
PubMed
Summary
This summary is machine-generated.

Tumor signals reprogram lymph node stromal cells, creating a pre-metastatic niche. These changes, involving marginal reticular cells and lymphatic endothelial cells, facilitate cancer cell metastasis.

Keywords:
Endothelial CellFibroblastStromal CellTumor Draining Lymph Nodemetastasispre-metastatic NichescRNAseq

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Area of Science:

  • Oncology
  • Immunology
  • Cell Biology

Background:

  • Metastatic dissemination depends on a receptive niche, influenced by primary tumor signals.
  • Lymph nodes, exposed to tumor signals via lymph, can be reprogrammed to support metastasis or immunosuppression.

Purpose of the Study:

  • To characterize tumor-driven transcriptomic changes in stromal cells within the tumor-draining lymph node.

Main Methods:

  • Single cell RNA sequencing was used to profile stromal cell reprogramming in lymph nodes of tumor-bearing and naive mice.

Main Results:

  • Tumor signals imprinted marginal reticular cells (MRCs) and floor lymphatic endothelial cells (fLECs) in the lymph node subcapsular sinus.
  • MRCs showed early desmoplastic CAF differentiation traits, fLECs activated chemoattractant pathways for cancer cell recruitment, and both populations exhibited metabolic and immune-modulating reprogramming.
  • These alterations were specific to tumor-draining lymph nodes and not observed during inflammatory challenges.

Conclusions:

  • Findings support the concept of pre-metastatic niche formation.
  • Identified stromal cell reprogramming offers potential targets for future therapeutic strategies.