Related Experiment Video
Updated: Jan 14, 2026
11:44
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
8.0K
Elucidating the Molecular Pathways of Long Noncoding RNA C6orf223 in Colorectal Cancer via microRNA Interactions and
Sara Tutunchi1, Amir-Reza Javanmard2, Ghodratollah Panahi3
1Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. utunchi.sara@sbmu.ac.ir.
Iranian Journal of Allergy, Asthma, and Immunology
|October 27, 2025
Summary
Long noncoding RNA C6orf223 is elevated in high-risk colorectal cancer (CRC), correlating with poor survival. This study highlights C6orf223 as a potential diagnostic and prognostic biomarker for CRC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Colorectal cancer (CRC) poses a significant global health challenge, with high-risk cases exhibiting aggressive behavior and unfavorable outcomes.
- Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development and progression, including in CRC.
- C6orf223 has emerged as a lncRNA of interest in the context of CRC pathogenesis.
Purpose of the Study:
- To investigate the expression patterns of C6orf223 in high-risk versus low-risk CRC.
- To evaluate the diagnostic and prognostic potential of C6orf223 as a biomarker for colorectal cancer.
- To explore the regulatory mechanisms, including microRNA interactions and epigenetic modifications, underlying C6orf223's role in CRC.
Main Methods:
- Differential gene expression analysis using RNA-sequencing data from CRC patients.
- Kaplan-Meier survival analysis and Receiver Operating Characteristic (ROC) curve analysis for prognostic and diagnostic assessment.
- Analysis of RNA methylation and mutation patterns, alongside correlation and pathway enrichment analyses to elucidate regulatory roles.
Main Results:
- C6orf223 expression was significantly upregulated in high-risk CRC compared to low-risk cases.
- Elevated C6orf223 levels were strongly associated with poorer overall survival in CRC patients.
- A diagnostic biomarker panel incorporating C6orf223 and specific microRNAs demonstrated high accuracy for CRC detection. Methylation and mutation analyses suggested mechanisms for C6orf223 stability and oncogenic function.
Conclusions:
- C6orf223 functions as an oncogenic lncRNA in colorectal cancer, potentially by sequestering tumor-suppressive microRNAs.
- C6orf223 holds significant promise as a valuable biomarker for the diagnosis and prognosis of colorectal cancer.
- Further investigation into lncRNA-microRNA interactions in CRC may unveil novel therapeutic strategies.
Related Concept Videos
01:22MicroRNAs
3.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.8K
01:22MicroRNAs
23.9K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.9K
02:39lncRNA - Long Non-coding RNAs
9.8K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.8K
02:39lncRNA - Long Non-coding RNAs
3.5K
3.5K