Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.3K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.3K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

977
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
977

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ligand-Unsupported Assembly of a Linear Penta-Tin Chain via Metal-Metal Donor-Acceptor Interactions.

Inorganic chemistry·2026
Same author

Mitochondrial NADP+-isocitrate dehydrogenase Idp1 involves CoQ biosynthesis in parallel with NAD kinase Pos5.

Bioscience, biotechnology, and biochemistry·2026
Same author

Mitochondrial NAD kinase Pos5 is required for CoQ biosynthesis in yeasts.

PloS one·2026
Same author

γ-Cyclodextrin Co-Ingestion Enhances the Bioavailability of Perilla Oil, Regardless of Inclusion Complex Formation.

International journal of molecular sciences·2025
Same author

3D-printed cuvette holder clip for a spectrophotometer.

BMC research notes·2025
Same author

Direct estimation of amylose and amylopectin in single starch granules by machine learning assisted Raman spectroscopy.

Carbohydrate polymers·2025

Related Experiment Video

Updated: Jan 6, 2026

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.9K

Posthandling Spectral Information Enhancement for Single Cell Raman Molecular Mapping Analysis.

Ankit Raj1,2, Nungnit Wattanavichean1,3, Makoto Kawamukai4

  • 1Department of Chemistry and Institute of Molecular Science, National Yang Ming Chiao Tung University, 1001 University Road, Hsinchu 300, Taiwan.

Analytical Chemistry
|October 27, 2025
PubMed
Summary

This study introduces an objective data analysis method for Raman microspectroscopy, improving molecular mapping of living cells by reducing human error and enabling the identification of minor Raman peaks.

More Related Videos

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells
10:37

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

Published on: August 22, 2025

998
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.5K

Related Experiment Videos

Last Updated: Jan 6, 2026

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.9K
A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells
10:37

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

Published on: August 22, 2025

998
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.5K

Area of Science:

  • Biomedical Engineering
  • Spectroscopy
  • Cell Biology

Background:

  • Label-free Raman microspectroscopy offers non-invasive molecular mapping of living cells.
  • Subjective data analysis and human error limit the accuracy and validity of current Raman spectral interpretation.
  • Objective postprocessing of large spectral datasets is needed for reliable cellular analysis.

Purpose of the Study:

  • To develop an objective data analysis scheme for postprocessing large Raman spectral datasets for molecular mapping of living cells.
  • To overcome limitations of subjective interpretation and human error in Raman microspectroscopy.
  • To enable precise mapping and identification of minor Raman peaks in cellular biochemical analysis.

Main Methods:

  • Spectral data denoising using low-rank approximation.
  • Objective background determination from external data points.
  • Background subtraction via Hypothetical Addition Multivariate Analysis with Numerical Differentiation (HAMAND) with an automated coefficient.

Main Results:

  • Successfully implemented an objective data analysis workflow for Raman spectral data.
  • Enabled the identification and precise mapping of previously indiscernible minor Raman peaks.
  • Demonstrated quantitative analysis of cellular components by isolating single-cell contributions from complex images.

Conclusions:

  • The presented objective analysis scheme enhances the accuracy of molecular mapping in living cells using Raman microspectroscopy.
  • This method facilitates the unambiguous identification of minor Raman peaks, improving biochemical analysis.
  • The improved workflow supports more reliable spectroscopic analysis of cellular structures and functions.