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Related Experiment Video

Updated: Jan 14, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Single-Molecule Array (Simoa) Technology as a Highly Sensitive Screening Tool for Protein-Protein Interactions.

Youngshin Lim^1,2, Maia Norman^3,4,5, Connie Chen¹

¹Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90048, United States.

|October 27, 2025

Summary

This summary is machine-generated.

Identifying weak transcription factor (TF) interactions is challenging. We adapted a sensitive Simoa assay for high-throughput screening, successfully identifying ARX TF interaction partners for neurodevelopmental disorder research.

Keywords:

ARX Digital ELISA Protein−protein interaction Screening Simoa Transcription factors

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Last Updated: Jan 14, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

Transcription factor (TF) interactions are typically weak and transient, complicating their study with standard methods.
This dynamic nature is crucial for cellular adaptation but poses significant technical hurdles for interaction analysis.

Purpose of the Study:

To adapt and validate an ultrasensitive single-molecule array (Simoa) assay for identifying transcription factor-transcription factor (TF-TF) interactions.
To screen a human TF library for interaction partners of ARX (aristaless-related homeobox), a TF implicated in neurodevelopmental disorders.

Main Methods:

Adapted a Simoa assay, a digital ELISA technique, for high-throughput screening of protein interactions.
Screened a library of human transcription factors to identify binding partners of ARX.

Main Results:

Successfully demonstrated the utility of Simoa technology for detecting TF-TF interactions.
Identified novel interaction partners for the ARX transcription factor.

Conclusions:

The adapted Simoa assay provides a powerful new tool for studying elusive TF-TF interactions.
This approach facilitates high-throughput screening for protein interaction partners, advancing research in areas like neurodevelopmental disorders.