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Ultra-low detection of SARS-CoV-2 Virus Like Particles (VLPs) with functionalized gold plasmonic nanoresonator array
Dipanjan Nandi1, Jiaxin Fan1, Seongdae Kang2
1Department of Electrical and Computer Engineering, University of Alberta, Edmonton, T6G 1H9, Alberta, Canada.
Abstract:
Localized surface plasmon resonance (LSPR) sensors have good potential for label-free non-invasive detection of biomolecules, healthcare diagnosis, disease monitoring, gas sensing, and food safety. For detection of low concentration small-sized bioanalytes (e.g., viruses, proteins, etc) the plasmonic field needs to be localized on a larger device surface area. Our study focuses on the optimization and fabrication of leaky Au nanoresonators based sensing platform for SARS-CoV-2 detection with uniform sensitivity over a 100 μm × 100 μm active sensing area. The Au nanoresonator array was optimized to detect 100 nm sized bioanalytes as it matches the size of SARS-CoV-2. The performance of the optimized design was tested with 100 nm sized polystyrene beads which demonstrated a sensitivity of 17.05 ± 3.25 nm/decade. The Au nanoresonators were functionalized with anti-SARS-CoV-2 antibody to detect SARS-CoV-2. Our experimental results demonstrate the best detection sensitivity of 1.32 ± 0.08 nm/decade and limit of detection of 1 VLP μL[Formula: see text].
