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Updated: Jan 14, 2026
High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
Published on: November 16, 2016
Phenotypic High-Throughput Screening and Drug Efficacy Test Against the Growth of Cryptosporidium parvum In Vitro by
Chenchen Wang1, Dongqiang Wang1, Haili Zhang2,3
1State Key Laboratory for the Diagnosis and Treatment of Severe Infectious Diseases, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Jilin, China.
Abstract:
An effective method to quantify parasite loads is key to evaluating anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening of drugs against Cryptosporidium parvum was previously infeasible using labor-intensive traditional assays. Here, we describe a simplified quantitative RT-PCR assay that directly uses cell lysates as templates, enabling high-throughput quantification of parasite loads. This assay can also be used to evaluate drug efficacy against the growth of C. parvum in vitro.
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