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Updated: Jan 14, 2026
Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection
Published on: September 14, 2019
Establishing TurboID Proximity Labeling and mAID Conditional Knockdown System in Cryptosporidium parvum
1State Key Laboratory for Animal Disease Control and Prevention Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Abstract:
Protein interaction networks and subcellular proteomes can provide key insights into the biological functions of protein complexes. Here, we describe a method for proximity-based labeling using TurboID to define protein interaction networks in Cryptosporidium parvum. In addition, we have developed an auxin-inducible degron (AID) labeling system for conditional protein degradation in C. parvum to facilitate functional studies. This chapter describes the procedures for generating miniTurbo or mAID-TIR1-tagged C. parvum strains and validating the TurboID and mAID systems for target genes.
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