Related Experiment Video
Updated: Jan 14, 2026
Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
Published on: April 16, 2019
The CXCL12-CXCR4 Axis Mediates Lymphocyte Immune Overactivation in IgG4-Related Chronic Rhinosinusitis
Jing Ding1,2, Li Cui3, Chengshuo Wang4,5
1Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Background:
IgG4-related chronic rhinosinusitis (IgG4-CRS) is a new clinical entity characterized by nasal lesions; however, its pathogenesis remains unclear. This study aimed to reveal single-cell transcriptomic changes in patients with IgG4-CRS via single-cell RNA sequencing (scRNA-seq) and illustrate its pathogenesis at the single-cell level.
Methods:
Nasal mucosal tissues from five patients with IgG4-CRS were used for unbiased scRNA-seq. Bioinformatic analysis was performed using these five samples and three published control samples. Immunohistochemical and multicolor immunofluorescence analyses were performed to validate the sequencing results.
Results:
A total of 49,063 cells and 11 sub-clusters were identified. CXCL12 secretion increased in endothelial cells and fibroblasts, and CXCR4 was upregulated in the immune cells of IgG4-CRS. Enhanced chemotaxis mediated by the CXCL12-CXCR4 axis recruits excess immune cells and overactivation. Compared with those in the control group, the immunocompetence of CD4+ T cells and cytotoxicity of CD8+ T cells were enhanced in the IgG4-CRS group, and B cells were more differentiated to secrete IgG-type plasma cells.
Conclusions:
Chemotaxis of the CXCL12-CXCR4 axis plays a role in the pathogenesis of IgG4-CRS by influencing both the immune and nonimmune cells of IgG4-CRS.
Related Concept Videos
01:24T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
02:06Allergic Reactions
01:27Allergic Drug Reactions
01:26Asthma-II: Pathophysiology and Classification
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
01:24B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
01:22T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...