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Real-time analysis and visualization of nanopore metagenomic samples with MARTi
Ned Peel1, Samuel Martin1, Darren Heavens1
1Earlham Institute, Norwich, NR4 7UZ, United Kingdom.
Genome Research
|October 27, 2025
Summary
Metagenomic Analysis in Real-Time (MARTi) is a new open-source software enabling rapid analysis of metagenomic data. It provides real-time insights into microbial communities and antimicrobial resistance, crucial for diagnostics and research.
Area of Science:
- Bioinformatics
- Metagenomics
- Genomic Sequencing
Background:
- Nanopore sequencing offers low-cost, portable, long-read, and real-time data generation for metagenomics.
- Current limitations in accessible, open-source bioinformatic tools hinder the full potential of real-time metagenomics.
Purpose of the Study:
- To introduce Metagenomic Analysis in Real-Time (MARTi), an open-source software for real-time metagenomic data analysis.
- To provide a user-friendly platform for real-time visualization and exploration of metagenomic data.
Main Methods:
- Developed MARTi, an open-source software with a browser-based graphical interface.
- Integrated multiple classification tools (BLAST, Centrifuge, Kraken2) with customizable parameters and user-defined databases.
- Validated performance using simulated and real-world metagenomic datasets.
Main Results:
- MARTi enables real-time taxonomic classification and antimicrobial resistance (AMR) gene identification.
- Demonstrated accurate read classification, taxon detection, and relative abundance estimation.
- Facilitated dynamic, real-time updates on microbial community composition and AMR profiles.
Conclusions:
- MARTi bridges the gap between nanopore sequencing and actionable insights for metagenomic studies.
- The software enhances the accessibility and functionality of real-time metagenomic analysis for diverse research applications.
- MARTi supports in-field diagnostics and large-scale metagenomic investigations.
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