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Actin cytoskeleton dynamics affect replication of human Metapneumovirus
Pamela Elizabeth Rodríguez1, Pedro Ignacio Gil2, Jorge Augusto Cámara1
1Laboratory of Influenza and other Respiratory Viruses, Virology Institute "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Córdoba, Argentina.
Scientific Reports
|October 28, 2025
Summary
Human Metapneumovirus (hMPV) replication is crucial in early stages. Disrupting actin microfilaments during this phase boosts hMPV protein expression and viral release.
Area of Science:
- Virology
- Cell Biology
- Infectious Diseases
Background:
- Human Metapneumovirus (hMPV) causes significant respiratory infections in vulnerable populations.
- The role of cellular structures, like the actin cytoskeleton, in hMPV replication remains largely uncharacterized.
Purpose of the Study:
- To investigate the involvement of the actin cytoskeleton in hMPV replication dynamics.
- To elucidate the impact of actin disruption on viral growth kinetics and protein expression.
Main Methods:
- Isolation and identification of hMPV (A2 genotype) in Vero cells.
- Analysis of viral growth curves and cytopathic effects.
- Treatment with Cytochalasin D to disrupt actin microfilaments during early infection.
Main Results:
- hMPV replication peaked between 48-72 hours post-infection (hpi).
- Maximal infected cell percentages and intracellular hMPV protein occurred early in the replication cycle.
- Disruption of actin microfilaments early in infection increased intracellular and extracellular virus yields.
Conclusions:
- The early phase of hMPV replication is critical for viral propagation.
- The actin cytoskeleton plays a significant role in hMPV replication, influencing viral protein expression and release.
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