Ultrastructural characterization of extracellular vesicles produced by Encephalitozoon cuniculi-infected B-1 cells

Herein, we analyzed the ultrastructure of the EVs released by E. cuniculi-infected B-1 cells or uninfected. Clusters of exosomes, also called intraluminal vesicles (ILVs), were surrounded by a membrane that was continuous with the plasma membrane of B-1 cells, characterizing the EVs known as multivesicular cargo (MVC). Migrasomes corresponded to clusters of exosomes located in the projection of the cell membrane. Ectosomes originated via the budding of the plasma membrane. To conclude, we showed that E. cuniculi-infected B-1 cells or B-1 CDP secrete large amounts of EVS especially after challenge with E. cuniculi.