Related Experiment Video
Updated: May 5, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Entecavir HPLC-MS/MS quantitation and optimized sample preparation using human plasma: Validation in a
Aims:
To determine the entecavir levels in human plasma using a quantitative high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technique.
Materials And Methods:
The developed method employed isotope-labeled internal standard, a high-throughput solid phase extraction process for sample preparation, and HPLC-MS/MS analysis using multiple reaction monitoring transitions in positive mode.
Results:
With a 0.025 - 10 ng/mL linear range, interday and intraday accuracy ranged from -3.4 to 5.3%; between-day and within-day precision was ≤ 7.3%. Stability, matrix effect, and recovery were all well within the acceptable criteria. A pharmacokinetic study of entecavir dispersible tablets at an oral dosage of 0.5 mg was successfully carried out using the validated method.
Conclusion:
The established HPLC-MS/MS method proved effective for quantitation of entecavir in human plasma.
More Related Videos
06:14Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients
Published on: October 15, 2017
08:54Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples
Published on: May 19, 2020
Related Concept Videos
Bioavailability Study Design: Healthy Subjects Versus Patients
Measurement of Bioavailability: Pharmacokinetic Methods
Measurement of Bioavailability: Pharmacodynamic Methods
Therapeutic Drug Monitoring: Drug Analysis Methods