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Published on: February 24, 2017
Biomimetic Mineralized Decellularized Adipose Tissue Accelerates Bone Repair by Immunomodulation and Dual
Tianlong Wang1,2, Zhiqing Liu1,2, Xinhui Wu1,2
1Center for Orthopaedic Science and Translational Medicine, Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, China.
This study developed a novel biomimetic mineralized scaffold from decellularized adipose tissue and tannic acid (TA@mDAT) to improve bone regeneration. The scaffold enhances bone formation, blood vessel growth, and reveals a potential mechanism involving the Notch1 signaling pathway.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Biomimetic mineralization offers a bottom-up approach for organic-inorganic hybrid materials for bone defect treatment.
- Current methods are insufficient for complex bone regeneration, which requires osteogenesis and microenvironment interaction.
Purpose of the Study:
- To develop a biomimetic mineralized scaffold (TA@mDAT) from decellularized adipose tissue (DAT) modified with tannic acid (TA).
- To investigate the scaffold's efficacy in promoting bone regeneration by mimicking microenvironment regulation and biomimetic strategies.
Main Methods:
- Decellularized adipose tissue (DAT) was modified with tannic acid (TA) and biomimetically mineralized to create TA@mDAT scaffolds.
- The scaffolds' mechanical properties, shape-memory effect, biocompatibility, immunomodulation, and bone regeneration efficacy were evaluated.
- The role of the Notch1 signaling pathway in bone regeneration was investigated by analyzing Dll4 and Hey1 expression.
Main Results:
- The TA@mDAT scaffold exhibited enhanced mechanical properties and shape-memory effect due to crosslinking between DAT and TA.
- The scaffold demonstrated excellent biocompatibility, immunomodulation, and efficacy in guiding bone regeneration via intramembranous and endochondral ossification.
- Enhanced H-type vessel formation and increased expression of Dll4 and Hey1, indicative of Notch1 pathway activation, were observed in regenerated bone.
Conclusions:
- The TA@mDAT scaffold is a promising material for bone regeneration, effectively triggering periosteal osteogenesis and promoting H-type vessel formation.
- The study highlights the Dll4-Notch1 signaling pathway as a potential key mechanism in the osteogenic process during bone regeneration.
