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Updated: Jan 13, 2026

Biomechanical Testing of Murine Tendons
Published on: October 15, 2019
The rectus femoris tendon demonstrates comparable ultimate stress to the patellar tendon: A paired biomechanical
Tomas Pineda1,2, Yash Sewpaul3,4, Vincent Morin5
1Universidad Andrés Bello, Hospital del Trabajador, Facultad de Medicina, Santiago, Chile.
Purpose:
The objective of this study was to compare the ultimate tissue strength of the rectus femoris (RF) and patellar tendons using paired soft-tissue grafts obtained from fresh organ donors, simulating clinical graft preparation.
Methods:
Fourteen paired RF and patellar tendon (PT) grafts were harvested from seven organ donors and tested within 12 h postmortem. The RF was folded and mounted as a double-stranded graft to simulate its clinical use. All grafts underwent uniaxial tensile testing using a standardised protocol. Primary outcomes included ultimate stress (MPa), load to failure (N) and elongation at failure (%). Data distribution was assessed with the Shapiro-Wilk test, and paired t-tests or Wilcoxon signed-rank tests were applied accordingly. A post hoc power analysis was conducted to determine the minimum detectable difference in stress with the given sample size.
Results:
Ultimate stress did not differ significantly between RF and PT grafts (46.4 ± 10.5 MPa vs. 52.9 ± 9.7 MPa, p = 0.184). RF grafts showed significantly lower load to failure (885.9 ± 52.3 N vs. 1278.7 ± 207.5 N, p < 0.001) and greater elongation (1.2 ± 0.2% vs. 0.2 ± 0.1%, p < 0.001).
Conclusion:
This study found that the double-stranded RF tendon exhibits ultimate tissue strength comparable to the patellar tendon, despite differences in load to failure and elongation. These results highlight the RF as a biomechanically viable graft option that merits further investigation. Given its consistent dimensions and soft-tissue-only harvest, the RF tendon represents a reproducible and potentially less invasive alternative for knee ligament reconstruction.
Level Of Evidence:
N/A.
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