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Dental Pulp Stem Cell-Derived Organoids: Advancing the Development of 3D Structures.
Loreto Lancia1, Fanny Pulcini1, Emanuela Mari2
1Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Cells
|October 28, 2025
Summary
Dental pulp stem cells (DPSCs) were used to create organoids that successfully differentiated into an osteogenic lineage. These dental pulp organoids formed mineralized matrices and expressed key bone-related markers.
Area of Science:
- Biomaterials Science
- Stem Cell Biology
- Regenerative Medicine
Background:
- Two-dimensional cell cultures lack in vivo complexity.
- Organoid technology overcomes limitations of traditional 2D cultures by mimicking tissue architecture.
- Dental pulp stem cells (DPSCs) offer high regenerative potential and accessibility for translational research.
Purpose of the Study:
- To generate and characterize dental pulp organoid models from DPSCs.
- To establish a robust in vitro model for translational studies in dentistry.
- To investigate the osteogenic differentiation potential of DPSC-derived organoids.
Main Methods:
- Dental pulp stem cells (DPSCs) were cultured to form organoids.
- Organoids were subjected to osteogenic differentiation.
- Cell viability (CCK-8 assay), mineralization (Alizarin red, Raman microspectroscopy), and marker expression (immunofluorescence) were analyzed.
Main Results:
- Alizarin red analysis showed increased calcium phosphate deposits in differentiated organoids.
- Raman microspectroscopy confirmed the presence of a hydroxyapatite-based biomaterial.
- Immunofluorescence revealed upregulated odonto/osteogenic markers (RUNX, OSX) and downregulated stemness markers.
Conclusions:
- DPSC-derived organoids successfully differentiated into an osteogenic lineage.
- The organoids formed a mineralized matrix containing hydroxyapatite.
- These findings support the use of dental pulp organoids for translational studies.
