Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Printable Core-Shell Multifunctional Particle for Light-Enhanced Nanomolar-Level Testosterone Point-of-Care Monitoring.

ACS sensors·2026
Same author

Does Peer Victimization Predict Loneliness? A Meta-Analysis of Longitudinal Studies.

Journal of youth and adolescence·2026
Same author

RNF213 Variants Associated With Periventricular Anastomosis Regression After Revascularization in Moyamoya Disease.

CNS neuroscience & therapeutics·2026
Same author

A CRY1 Interactor eIF3G1 Negatively Regulates Root Growth Under Blue Light in <i>Arabidopsis</i>.

Plants (Basel, Switzerland)·2026
Same author

Combinatorial optimization enhanced by shallow quantum circuits with 104 superconducting qubits.

National science review·2026
Same author

A deep learning system for non-invasive breast cancer diagnosis with multimodal data.

Nature biomedical engineering·2026
Same journal

Correction: Sutthanont et al. Effectiveness of Herbal Essential Oils as Single and Combined Repellents Against <i>Aedes aegypti</i>, <i>Anopheles dirus</i> and <i>Culex quinquefasciatus</i> (Diptera: Culicidae). <i>Insects</i> 2022, <i>13</i>, 658.

Insects·2026
Same journal

A Taxonomic Revision of the East Mediterranean Species of the <i>Crematogaster scutellaris</i> Complex (Hymenoptera: Formicidae).

Insects·2026
Same journal

Structural Characteristics for the Interaction of 1-Benzyl-2-Methylbenzimidazoles as Insect Growth Regulators and Juvenile Hormone Binding Protein.

Insects·2026
Same journal

Structure of Epigeic and Arboreal Ant Communities in Forest Fragments Within Agricultural Landscapes of the Brazilian Cerrado.

Insects·2026
Same journal

Insects as an Alternative Protein Source: A Sustainable Approach to Future Food Security.

Insects·2026
Same journal

Carpet Beetle Species (Coleoptera: Dermestidae) in Austrian Heritage Interiors and Their European Distributions.

Insects·2026
See all related articles

Related Experiment Video

Updated: Jan 13, 2026

Author Spotlight: Evaluation of Entomopathogenic Fungi in Wild Monochamus alternatus Populations for Biocontrol Applications in Forest Wood Borers
06:58

Author Spotlight: Evaluation of Entomopathogenic Fungi in Wild Monochamus alternatus Populations for Biocontrol Applications in Forest Wood Borers

Published on: September 29, 2023

1.3K

Comparative Transcriptomics Unveils Pathogen-Specific mTOR Pathway Modulation in Monochamus alternatus Infected with

Haoran Guan1, Jinghong He1,2, Chuanyu Zhang1

  • 1Basic Forestry and Proteomics Research Center, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, School of Future Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Insects
|October 28, 2025
PubMed
Summary
This summary is machine-generated.

Controlling pine wilt disease requires understanding how fungi infect pine trees. Targeting the mTOR pathway enhances Beauveria bassiana

Keywords:
Beauveria bassianaMetarhizium anisopliaeMonochamus alternatusdual RNA-seqmTOR pathwaypine wilt disease

More Related Videos

Establishment of a Chilli Thrips (Scirtothrips dorsalis Hood) Rearing System for Virulence Screening of Entomopathogenic Fungi
11:35

Establishment of a Chilli Thrips (Scirtothrips dorsalis Hood) Rearing System for Virulence Screening of Entomopathogenic Fungi

Published on: July 18, 2025

851
IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae
08:22

IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae

Published on: January 15, 2020

6.6K

Related Experiment Videos

Last Updated: Jan 13, 2026

Author Spotlight: Evaluation of Entomopathogenic Fungi in Wild Monochamus alternatus Populations for Biocontrol Applications in Forest Wood Borers
06:58

Author Spotlight: Evaluation of Entomopathogenic Fungi in Wild Monochamus alternatus Populations for Biocontrol Applications in Forest Wood Borers

Published on: September 29, 2023

1.3K
Establishment of a Chilli Thrips (Scirtothrips dorsalis Hood) Rearing System for Virulence Screening of Entomopathogenic Fungi
11:35

Establishment of a Chilli Thrips (Scirtothrips dorsalis Hood) Rearing System for Virulence Screening of Entomopathogenic Fungi

Published on: July 18, 2025

851
IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae
08:22

IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae

Published on: January 15, 2020

6.6K

Area of Science:

  • Entomology
  • Plant Pathology
  • Molecular Biology

Background:

  • Pine wilt disease (PWD), transmitted by Monochamus alternatus (JPS), threatens global pine forests.
  • Entomopathogenic fungi Beauveria bassiana (Bb) and Metarhizium anisopliae (Ma) are eco-friendly biocontrol agents but face challenges in field efficacy due to limited understanding of host-pathogen interactions.

Purpose of the Study:

  • To compare the transcriptional responses of JPS to Bb and Ma infections at 48 hours post-infection.
  • To elucidate the molecular mechanisms underlying host-pathogen specificity in PWD biocontrol.
  • To identify potential targets for enhancing the efficacy of fungal biocontrol agents.

Main Methods:

  • Dual RNA-sequencing was used to analyze gene expression in JPS and infecting fungi.
  • Bioinformatic analyses identified differentially expressed genes and pathways.
  • Functional validation involved mTOR inhibition using chemical inhibitors and RNA interference (RNAi) in JPS.

Main Results:

  • Bb infection induced autophagy and modulated JPS carbohydrate metabolism, leading to tissue degradation.
  • Ma infection co-opted host metabolic pathways for biosynthesis and triggered stronger JPS immune responses.
  • The mTOR signaling pathway was identified as a key regulator of differential host responses.
  • mTOR inhibition selectively enhanced Bb-induced mortality in JPS but did not affect Ma virulence.

Conclusions:

  • Distinct infection strategies employed by Bb and Ma lead to specific host responses in JPS.
  • The mTOR signaling pathway plays a crucial role in regulating JPS susceptibility to entomopathogenic fungi.
  • Targeting mTOR represents a promising strategy to improve the field performance of Bb as a biocontrol agent for PWD.