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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

101.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
101.9K

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Related Experiment Video

Updated: Apr 13, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

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Real-World Technical Hurdles of ctDNA NGS Analysis: Lessons from Clinical Implementation.

Simon Cabello-Aguilar1,2, Julie A Vendrell1, Jérôme Solassol1,3

  • 1Laboratoire de Biologie des Tumeurs Solides, CHU Montpellier, Université de Montpellier, 34295 Montpellier, France.

Diseases (Basel, Switzerland)
|October 28, 2025
PubMed
Summary

Next-generation sequencing of circulating tumor DNA (ctDNA) offers a minimally invasive cancer detection method. Improvements in detection limits and bioinformatics can enhance its clinical utility and accuracy.

Area of Science:

  • Oncology
  • Genomics
  • Molecular Diagnostics
Keywords:
NGSctDNAliquid biopsyprecision oncology

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Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
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Last Updated: Apr 13, 2026

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Background:

  • Circulating tumor DNA (ctDNA) analysis via next-generation sequencing (NGS) provides a minimally invasive method for cancer detection and monitoring.
  • Liquid biopsy using ctDNA offers real-time genomic insights into tumor heterogeneity, aiding early detection and treatment response assessment.
  • Clinical implementation faces challenges due to lack of standardized ctDNA quantification and limited sensitivity for low-frequency variants.