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A Biofuel Cell for Electricity Generation from Biomass-Derived Cellobiose.

Piyanut Pinyou1, Peeranat Jatooratthawichot1,2, Luciranon Sribrahma1,2

  • 1School of Chemistry, Institute of Science, Suranaree University of Technology, 111 University Ave., Muang, Nakhon Ratchasima 30000, Thailand.

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A novel bioanode utilizes two enzymes for efficient biofuel cell performance. This enzyme-based system effectively converts cellobiose into electricity, showing promise for sustainable energy applications.

Keywords:
biofuel cellbiomasscellobioseglucose oxidaseβ-glucosidase

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Area of Science:

  • Biotechnology and Bioengineering
  • Electrochemistry
  • Enzyme Catalysis

Background:

  • Biofuel cells offer a sustainable energy alternative.
  • Enzyme-catalyzed reactions are crucial for bioanode development.
  • Efficient conversion of biomass-derived fuels is essential.

Purpose of the Study:

  • To develop and optimize a novel bioanode using a two-enzyme cascade.
  • To investigate the performance of the bioanode with cellobiose and biomass hydrolysates.
  • To explore the impact of enzyme kinetics and pretreatment methods on biofuel cell efficiency.

Main Methods:

  • Fabrication of a glassy carbon electrode modified with beta-glucosidase (TxGH116) and glucose oxidase (GOx) entrapped in an osmium redox polymer.
  • Investigation of enzyme kinetic parameters at various temperatures.
  • Optimization of glucose oxidase loading and enzyme/polymer ratio.
  • Coupling the bioanode with a horseradish peroxidase (HRP) biocathode.
  • Testing biofuel cell performance using cellobiose and pretreated sugarcane hydrolysates.

Main Results:

  • The bioanode successfully catalyzed the conversion of cellobiose to D-glucose and then to gluconolactone, generating electrons.
  • Kinetic parameters for both enzymes were determined, and optimal conditions for GOx were established.
  • Alkaline pretreatment of sugarcane biomass proved more effective than phosphoric acid pretreatment.
  • Addition of TxGH116 beta-glucosidase significantly enhanced current generation, even with commercial cellulase.

Conclusions:

  • A robust two-enzyme bioanode system was successfully developed for biofuel cells.
  • The bioanode demonstrates efficient electricity generation from cellobiose and biomass hydrolysates.
  • Optimized enzyme loading, pretreatment strategies, and enzyme addition are key to maximizing biofuel cell performance.