Universal Platform Based on Carbon Nanotubes Functionalised with Carboxylic Acid Groups for Multi-Analyte Enzymatic...

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Biotechnology

Background:

• Development of sensitive and selective biosensors is crucial for health and food monitoring.
• Carbon nanotubes offer unique electrochemical properties for biosensing applications.
• Oxygen-sensitive platforms can enable multi-analyte detection.

Purpose of the Study:

• To create a carbon nanotube-based electrochemical platform for parallel enzymatic biosensing.
• To functionalize carbon nanotubes with carboxylic acid groups (CNT-COOH) for enhanced electrode attachment.
• To develop a multi-analyte biosensor for simultaneous detection of glucose and lactate.

Main Methods:

• Fabrication of nanostructured gold electrodes modified with 4-aminothiophenol and CNT-COOH.
• Immobilization of glucose oxidase, lactate oxidase, glutamate oxidase, and tyrosinase onto electrodes.
• Electrochemical characterization and analysis of analytes in blood plasma samples.
• Incorporation of catalase to mitigate hydrogen peroxide accumulation.

Main Results:

• Carboxylic-acid-functionalised carbon nanotubes were successfully attached horizontally onto gold electrodes.
• The multifunctional biosensor achieved simultaneous and independent measurement of glucose and lactate.
• Catalase addition significantly extended the linear detection range by managing hydrogen peroxide.
• The biosensor demonstrated reliable performance in complex biological samples like blood plasma.

Conclusions:

• The developed CNT-COOH platform is a viable oxygen-sensitive electrochemical tool for multi-analyte biosensing.
• The integration of catalase enhances biosensor performance and broadens detection capabilities.
• This technology holds significant potential for advancing health diagnostics and food safety analysis.