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A Highly Sensitive Fluorescent Sensor Based on Carbon Dots and Gold Nanoparticles for Carbaryl Through the Inner
Yan Lu1, Chengqi Bao1, Minghui Yang1
1Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Biosensors
|October 28, 2025
Summary
A novel fluorescent sensor using carbon dots (CDs) and gold nanoparticles (AuNPs) detects carbaryl pesticide. This method leverages the inner filter effect (IFE) and acetylcholinesterase (AChE) activity for sensitive and specific carbaryl detection in food samples.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Materials Science
Background:
- Pesticide detection is crucial for food safety and environmental monitoring.
- Existing methods for carbaryl detection can be complex and time-consuming.
- Development of sensitive and selective sensing platforms is needed.
Purpose of the Study:
- To develop a highly sensitive fluorescent sensing platform for carbaryl detection.
- To utilize the inner filter effect (IFE) between carbon dots (CDs) and gold nanoparticles (AuNPs).
- To incorporate acetylcholinesterase (AChE) for specific carbaryl inhibition-based detection.
Main Methods:
- Fabrication of a fluorescent sensor using carbon dots (CDs) and gold nanoparticles (AuNPs).
- Exploitation of the inner filter effect (IFE) for signal generation.
- Utilizing acetylcholinesterase (AChE) inhibition by carbaryl to modulate fluorescence.
- Optimization of analytical conditions for carbaryl detection.
Main Results:
- The sensor exhibited a significant fluorescence quenching due to the IFE between CDs and AuNPs.
- Carbaryl inhibited AChE activity, preventing thiocholine formation and AuNP aggregation, thus maintaining fluorescence quenching.
- A wide detection range of 0.1–200 ng/mL with a low limit of detection (LOD) of 0.05 ng/mL was achieved.
- The sensor demonstrated high accuracy and precision for carbaryl detection in strawberry samples (97.5%-101.1% recovery, <5% RSD).
Conclusions:
- A sensitive and selective fluorescent sensor for carbaryl detection was successfully developed.
- The sensor effectively utilizes the synergistic effects of CDs, AuNPs, and AChE inhibition.
- The proposed method shows great potential for practical application in food safety analysis.
