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Related Experiment Video

Updated: May 2, 2026

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres
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Biofunctional Carboxymethyl Chitosan Hydrogel Incorporating Hyaluronic Acid and RGD Peptides for Accelerated Wound

Shuyue Wang1, Qing Yang2, Jiren Xu1

  • 1Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Gels (Basel, Switzerland)
|October 28, 2025
PubMed
Summary

A novel carboxymethyl chitosan hydrogel enhanced with hyaluronic acid and RGD peptides shows significant potential for wound healing. This advanced wound dressing promotes cell viability, proliferation, and migration, accelerating tissue repair for chronic wounds.

Keywords:
RGD peptidecarboxymethyl chitosanhyaluronic acidhydrogelwound repair

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Carboxymethyl chitosan (CMC)-based hydrogels are promising for wound dressings due to biocompatibility.
  • Need for advanced wound healing materials to address chronic and refractory wounds.
  • Functionalization with hyaluronic acid (HA) and RGD peptides can enhance biological activity.

Purpose of the Study:

  • To fabricate and characterize a novel CMC-based hydrogel functionalized with HA and RGD peptides.
  • To evaluate the structural properties, biocompatibility, and wound-healing potential of the developed hydrogel.
  • To determine the optimal formulation for enhanced wound repair.

Main Methods:

  • Fabrication of four hydrogel variants (CMC, CMC-HA, CMC-RGD, CMC-HA-RGD) using EDC/NHS crosslinking.
  • Characterization using Scanning Electron Microscopy (SEM), Zeta potential, Fourier-transform infrared spectroscopy (FTIR), and Thermogravimetric Analysis (TGA).
  • In vitro evaluation of cell viability (CCK-8 assay), proliferation (Ki-67 immunofluorescence), and migration (scratch assay) using fibroblasts.

Main Results:

  • The optimal formulation (1% CMC, 0.9% HA, 0.02 mg/mL RGD) exhibited a porous structure (100-400 μm) and colloidal stability (|ζ| > 30 mV).
  • FTIR confirmed successful crosslinking and integration of HA and RGD. TGA showed enhanced thermal stability.
  • CMC-HA-RGD hydrogel significantly improved fibroblast viability (p < 0.05), proliferation, and migration compared to other groups, indicating accelerated wound repair.

Conclusions:

  • The developed CMC-HA-RGD hydrogel possesses favorable physicochemical and biological properties for wound healing.
  • Synergistic modification with HA and RGD significantly enhances cellular migration and facilitates wound repair.
  • This novel hydrogel holds strong potential as an advanced wound dressing for chronic and refractory wounds.