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Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat
Published on: February 7, 2011
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Performing Dual Glucose Clamp Experiments in Sedated Farm Swine: A Practical Method.
Marc C Torjman1, Winston C Hamilton1, Katherine Dillon1
1Department of Anesthesiology and Perioperative Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Methods and Protocols
|October 28, 2025
Summary
This study presents a practical method for hyperinsulinemic-euglycemic clamp experiments in swine, improving insulin sensitivity measurement in large animals. The developed technique ensures animal welfare and maintains stable glucose levels, potentially reducing experimental costs.
Area of Science:
- Veterinary Medicine
- Physiology
- Metabolic Research
Background:
- The hyperinsulinemic-euglycemic clamp is the gold standard for assessing insulin sensitivity in large animals.
- Existing methods can be resource-intensive and require specialized infrastructure.
Purpose of the Study:
- To develop and validate a practical, concurrent glucose clamp method for farm swine.
- To establish a reliable protocol for measuring insulin sensitivity in large animal models.
Main Methods:
- A novel sling-based setup was used for sedated swine undergoing concurrent glucose clamp experiments.
- Customized equipment and surgical procedures for central venous access were detailed.
- A standardized protocol involving specific anesthetic, infusion rates, and monitoring was executed over 24 studies.
Main Results:
- The protocol successfully maintained mean blood glucose levels between 75.7 and 87.8 mg/dL (CV 3.17%).
- Implanted infusaports remained functional for extensive blood sampling (1454 samples) without infection.
- Animals remained comfortable and physiologically stable (SpO2, heart rate) throughout the procedures.
Conclusions:
- The developed hyperinsulinemic-euglycemic clamp method is practical, safe, and effective for large animal research.
- This protocol can enhance the efficiency and reduce the cost of insulin sensitivity studies in swine.
- The technique holds potential for optimizing resource utilization in metabolic research.
