Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Equipments Used to Measure Body Temperature01:13

Equipments Used to Measure Body Temperature

1.7K
Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
1.7K
Temperature Measurement Sites01:14

Temperature Measurement Sites

3.2K
A thermometer measures body temperature. The common sites for measuring body temperature are the oral cavity, axillary region, temporal artery, and skin surface, such as the forehead, abdomen, and axilla. True core body temperature is assessed in the rectum, tympanic membrane, pulmonary artery, esophagus, and urinary bladder.
Oral: When assessing oral temperature, the thermometer tip should be placed under the tongue in the posterior sublingual pocket. It offers accurate readings and can be...
3.2K
Assessing Body Temperature - Temporal Artery01:19

Assessing Body Temperature - Temporal Artery

1.0K
Here is a stepwise guide to assessing the body temperature at the temporal artery using a temporal artery thermometer
Step 1: Perform hand hygiene and don a fresh pair of gloves to prevent cross-infection and ensure patient safety.
Step 2: Explain the procedure to the patient to establish trust. Clear communication establishes trust with the patient, ensures they understand what to expect, promotes cooperation, and enhances comfort during the procedure.  
Step 3: Assess the patient's...
1.0K
Assessing Body Temperature - Oral01:14

Assessing Body Temperature - Oral

1.4K
Here are the steps to accurately measure oral temperature using an electronic thermometer:
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
1.4K
Assessing Body Temperature - Axilla01:14

Assessing Body Temperature - Axilla

1.2K
Procedural Guide for Assessing Axillary Body Temperature using a Digital Thermometer:
Step 1: Perform hand hygiene and put on clean gloves to maintain infection control and prevent cross-contamination.
Step 2: Prepare the patient by explaining the procedure to ensure understanding and cooperation. Ensure privacy, expose the axilla, and inform the patient that minimal movement is crucial for an accurate reading.
Step 3: Adjust the patient’s clothing to expose only the axilla. It minimizes...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond compliance: How accreditation can strengthen trust in science and research infrastructure.

Journal of clinical and translational science·2026
Same author

Research capacity. Enabling the genomic revolution in Africa.

Science (New York, N.Y.)·2014
Same author

Docosahexaenoic acid reduces inflammation and joint destruction in mice with collagen-induced arthritis.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2013
Same author

Mammary tumor modifiers in BALB/cJ mice heterozygous for p53.

Mammalian genome : official journal of the International Mammalian Genome Society·2007
Same author

Transgenic E2F1 expression in the mouse brain induces a human-like bimodal pattern of tumors.

Cancer research·2007
Same author

A novel CRM1-dependent nuclear export signal in adenoviral E1A protein regulated by phosphorylation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2006

Related Experiment Video

Updated: Jan 13, 2026

An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices
08:44

An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices

Published on: August 21, 2020

7.8K

Indicator Tubes: A Novel Solution for Monitoring Temperature Excursions in Biobank Storage.

Patrick J Catterson1, Tyler T Olson1, Margaret B Penno1

  • 1The Johns Hopkins BioBank, Genetic Resources Core Facility, Department of Genetic Medicine, Johns Hopkins University School of Medicine, 600 N. Wolfe St., 1017 Blalock, Baltimore, MD 21287, USA.

Methods and Protocols
|October 28, 2025
PubMed
Summary

New frozen indicator tubes provide a visual backup for cryogenic storage. These cost-effective devices detect temperature excursions, ensuring the integrity of sensitive biological materials during storage.

Keywords:
biobankingbiobanking quality assurancecellular viabilitylong-term cryogenic storagespecimen integritytemperature excursion detectiontemperature indicators

More Related Videos

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
09:32

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

Published on: February 4, 2013

21.4K
Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED
09:32

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED

Published on: May 7, 2013

19.5K

Related Experiment Videos

Last Updated: Jan 13, 2026

An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices
08:44

An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices

Published on: August 21, 2020

7.8K
LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
09:32

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

Published on: February 4, 2013

21.4K
Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED
09:32

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED

Published on: May 7, 2013

19.5K

Area of Science:

  • Cryobiology and sample preservation science.

Background:

  • Cryogenic storage is essential for biological material viability.
  • Existing electronic monitoring systems may fail to detect transient thaw-refreeze events.
  • Such undetected events pose significant risks to sample quality and integrity.

Purpose of the Study:

  • To develop and validate visual indicator tubes as a backup monitoring system for cryogenic storage.
  • To provide a cost-effective solution for detecting temperature deviations.
  • To enhance the reliability of cryopreservation protocols.

Main Methods:

  • Development of two types of indicator tubes: an aqueous dye solution and an ethanol-based solution for ultra-low temperatures.
  • The aqueous dye method uses dye immobilization and dispersion to signal thawing.
  • The ethanol-based method is calibrated to detect specific temperature rises (≥10 °C for ≥15 minutes).

Main Results:

  • The aqueous dye tubes provide a clear visual cue of partial or complete thawing.
  • The ethanol-based tubes successfully indicate thaw events meeting critical stability thresholds.
  • Both methods offer a reliable, immediate visual confirmation of temperature breaches.

Conclusions:

  • Frozen indicator tubes serve as an effective, low-cost backup to electronic monitors in cryogenic storage.
  • These indicators enhance confidence in the long-term preservation of sensitive biological materials.
  • The visual signaling system improves the overall safety and reliability of cryopreservation practices.