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Published on: November 30, 2016
Tremella-derived carbon dots-based UV-vis/fluorescent dual-output sensing platform for multiplex miRNA detection
Jing-Lin He1, Xiaohui Wu1, Shiqing Liao1
1Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation & Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Pharmaceutical Engineering, Changsha University of Science and Technology, Changsha, 410114, China.
None:
In this study, we present a novel ultraviolet-visible/fluorescent dual-output sensing platform for the multiplexed detection of microRNAs (miRNAs) in tumor cells. For the first time, carbon dots (CDs) were synthesized from Tremella via a one-step hydrothermal process and integrated with poly(A)-functionalized gold nanoparticles (AuNPs) and DNA nanohooks generated through hybridization chain reaction (HCR). In the absence of target miRNA, HCR triggers the formation of DNA nanohooks, leading to AuNP aggregation and a visible color change from red to colorless, while the CDs emit strong fluorescence. Upon target binding, HCR is inhibited, preserving AuNP dispersion and causing fluorescence quenching due to CD-AuNP proximity. This dual-signal response enables reliable, background-minimized detection. By simply altering the recognition sequences, the platform allows simultaneous detection of multiple miRNAs, such as miR-222, miR-21, and miR-122. The use of Tremella-derived CDs offers a sustainable, biocompatible alternative to conventional fluorescent materials. This platform demonstrates excellent sensitivity, specificity, and versatility, showing great potential for miRNA-based cancer diagnostics and high-throughput clinical analysis.
