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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin shRNA
Published on: February 12, 2020
Critical lncRNA screening and ceRNA network Construction in bovine skeletal muscle cells and intramuscular adipocytes
Dongmei Yang1, Yaping Song1, Hongfang Gong1
1College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; Key Laboratory of Ruminant Molecular Cell Breeding, Ningxia Hui Autonomous Region, Yinchuan 750021, China.
Abstract:
Intramuscular fat (IMF) is a critical indicator affecting meat quality, whose formation and deposition are influenced not only by the cellular microenvironment but also by the coordinated regulation of multiple genes. During this process, long non-coding RNAs (lncRNAs) can competitively bind miRNAs through a molecular sponge mechanism, thereby participating in the regulation of adipocyte growth and further influencing IMF deposition. Therefore, lncRNA sequencing was performed using a bovine skeletal muscle-intramuscular adipocyte co-culture model. Analysis revealed 99 differentially expressed (DE) lncRNAs. To further investigate the regulatory functions of lncRNAs, antisense,cis-acting, andtrans-acting target prediction analyses were performed. These analyses identified 4 antisense, 7cis-acting, and 98trans-acting DE lncRNA-mRNA interaction pairs. Subsequently, a competing endogenous RNA (ceRNA) network encompassing 260 potential interactions was constructed. Among these, the 20 highest-confidence interactions involved 8 DE-lncRNAs, 11 miRNAs, and 7 mRNAs. These findings establish a foundation for elucidating molecular mechanisms of lncRNA-mediated IMF deposition regulation in cattle via ceRNA pathways.
