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Updated: Jan 13, 2026

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel
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Verbascoside Suppresses Epithelial-Mesenchymal Transition and Mitochondrial Biogenesis by Targeting Anti-senescence

Hsing-Chia Mai1,2,3, Pei-Fang Hsieh4,5, Chun-Hsien Wu1,3,4,6

  • 1Department of Urology, E-Da Cancer Hospital, Kaohsiung, Taiwan, R.O.C.

Cancer Genomics & Proteomics
|October 28, 2025
PubMed
Abstract

Insights

Verbascoside, a natural compound, combats aggressive prostate cancer by reducing cell migration, oxidative stress, and senescence. It enhances mitochondrial function and inhibits key pathways driving cancer progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Castration-resistant prostate cancer (CRPC) is an aggressive malignancy.
  • Verbascoside, a phenylethanoid glycoside, shows therapeutic promise for CRPC.
  • Mechanisms underlying verbascoside's effects require elucidation.

Purpose of the Study:

  • Investigate verbascoside's antisenescence and anti-metastatic effects in CRPC.
  • Elucidate verbascoside's impact on epithelial-mesenchymal transition (EMT), oxidative stress, and mitochondrial biogenesis.
  • Analyze verbascoside's modulation of senescence-associated secretory phenotype (SASP) and cell cycle regulators.

Main Methods:

  • CRPC cell models treated with verbascoside.
  • Western blotting and ELISA to assess EMT markers, oxidative stress, mitochondrial regulators, and cytokines.
  • Analysis of p38 MAPK, p16, p21, p27, and Rb for senescence and proliferation.

Main Results:

  • Verbascoside inhibited EMT, reduced oxidative stress, and enhanced mitochondrial biogenesis.
  • Suppressed secretion of proinflammatory cytokines (IL-6, IL-8, IL-1).
  • Decreased p38 MAPK activation and down-regulated p16, p21, p27, and Rb, reducing senescence and proliferation.

Conclusions:

  • Verbascoside attenuates CRPC progression via modulation of EMT, oxidative stress, mitochondrial function, and senescence pathways.
  • Highlights verbascoside's potential in targeting senescence in aggressive prostate cancer.
  • Provides a basis for future CRPC management strategies targeting senescence.
Keywords:
Verbascosideanti-senescencecastration-resistant prostate cancerepithelial–mesenchymal transitionmitochondrial biogenesis

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