Related Experiment Video
Updated: Jan 13, 2026
08:08
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
Published on: February 27, 2015
16.9K
Global Profiling of Lysine Lactylation in Prostate Cancer Cells
Jae Yong Kim1, Hyunchae Sim1, Ann-Yae Na1
1School of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea.
Cancer Genomics & Proteomics
|October 28, 2025
Summary
Lysine lactylation (Kla) is a newly discovered modification in prostate cancer (PC). This study reveals its landscape and potential role in PC progression, suggesting Kla as a therapeutic target.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Lysine lactylation (Kla) is a post-translational modification regulated by lactate.
- Kla's role in prostate cancer (PC) is not well understood, despite its involvement in other cancers.
Purpose of the Study:
- To profile lysine lactylation (Kla) in prostate cancer (PC).
- To explore the mechanisms of Kla in PC progression.
Main Methods:
- Global Kla profiling in PC-3M prostate cancer cells using affinity enrichment and LC-MS/MS.
- Bioinformatics analyses to determine functional roles of Kla-modified proteins.
Main Results:
- Identified 681 Kla sites on 379 proteins, primarily in nuclear and cytoplasmic proteins.
- Kla is involved in mRNA splicing, chromatin organization, and glycolysis/gluconeogenesis.
- Reduced expression of SIRT3, SIRT5, and SIRT6 in PC-3M cells may contribute to elevated Kla levels.
Conclusions:
- This study provides the first comprehensive landscape of Kla in prostate cancer (PCa).
- Kla may play a regulatory role in PCa progression.
- Kla is a potential therapeutic target for prostate cancer intervention.
