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OIP5-AS1 Positively Regulates the Proliferation of Colorectal Cancer Cells by Repressing Apoptosis
Yojiro Kotake1,2, Yoshinobu Tanigawa3, Hikari Ikenaga3
1Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Fukuoka, Japan; ykotake@fuk.kindai.ac.jp.
Anticancer Research
|October 28, 2025
Summary
The long noncoding RNA OIP5-AS1 promotes colorectal cancer (CRC) cell growth by suppressing apoptosis. Combining OIP5-AS1 knockdown with 5-fluorouracil (5-FU) treatment effectively inhibits CRC cell proliferation.
Area of Science:
- Molecular Biology
- Cancer Research
- Genetics
Background:
- OIP5-AS1 is a long noncoding RNA implicated in promoting cancer cell proliferation, invasion, and migration across various cancer types.
- This study investigates the specific role of OIP5-AS1 in colorectal cancer (CRC) progression.
Purpose of the Study:
- To elucidate the mechanism by which OIP5-AS1 promotes colorectal cancer cell proliferation.
- To assess the therapeutic potential of targeting OIP5-AS1 in combination with 5-fluorouracil (5-FU).
Main Methods:
- Human CRC cell lines (HCT116 and DLD-1) were utilized.
- Small interfering RNAs (siRNAs) were employed to silence OIP5-AS1 expression.
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and flow cytometry were used to analyze gene expression, cell cycle, and apoptosis.
Main Results:
- Silencing OIP5-AS1 significantly inhibited CRC cell proliferation, including three-dimensional growth.
- OIP5-AS1 knockdown increased apoptosis without affecting the cell cycle.
- The expression of OIP5-AS1 was upregulated by the anticancer drug 5-FU.
- Combined treatment with 5-FU and OIP5-AS1 knockdown markedly suppressed HCT116 cell proliferation.
Conclusions:
- OIP5-AS1 promotes colorectal cancer cell proliferation by inhibiting apoptosis.
- Downregulating OIP5-AS1 in conjunction with 5-FU treatment offers a more effective strategy for inhibiting CRC cell proliferation.
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